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FIG. 2. Effects of treating dams with TCDD on the expression of StAR and PBR in fetal testis. A, Testicular and adrenal (Adr.) expression of StAR and PBR mRNAs in the fetuses (GD20) of dams treated with TCDD and vehicle at GD15. C, Control; T, TCDD. B, Amount of StAR mRNA expressed in fetal testes. Each bar represents the mean ± SD of six fetuses, two of which were removed from three different parents. A significant difference is shown by an asterisk (P < 0.05). C, Expression of StAR protein detected by immunoblotting in fetal testis is shown. The testis mitochondrial fraction (30 µg protein) of fetuses from four different dams was electrophoresed and blotted. The testes of four to nine fetuses from a dam were pooled and the mitochondrial fraction was prepared.