TABLE 1. Primers used in the PCR for the quantification of mRNA

Target mRNA Forward primer (5'–3') Reverse primer (5'–3') Size of product (bp) Annealing temp. (C) Extention cycles Accession no.1
CYP1A1 CCATGACCAGGAACTATGGG TCTGGTGAGCATCCAGGACA 341 55 322 E00778
StAR CATCCAGCAAGGAGAGGAAG CGTGAGTTTGGTCTTTGAGG 496 64 233 U76419
PBR AAGAGCTGGGAGGTTTCACA CCAGGCCAGGTAAGGATACA 224 57 27 J05122
CYP11A1 TCAAAGCCAGCATCAAGGAG GCAGCCTGCAATTCATACAG 473 60 264 M22615
CYP11B1 GTCTATAAACATTCAGTCCAA ATCTCGGATATGACACTCC 324 60 35 X15431
CYP17 GTCACTGTGTGATATGATGCAGGC TTCAGGCATGAACTGATCTGCCT 793 64 26 M31681
CYP19 GCTTCTCATCGCAGAGTATCCGG CAAGGGTAAATTCATTGGGCTGG 266 60 38 M33986
3ß-HSD GCCCATGTACATTTATGGGG CCCTTTCTGTCACTGAGACT 574 62 25 M38179
17ß-HSD GTAGTGCTCATTACCGGTTG CACCCAGTACATCCACTCGACC 334 55 31 X98038
ER{alpha} AATTCTGACAATCGACGC CTTCAACATTCTCCCTCCTC 342 60 33 Y00102
ERß CTCGGGGTCTGAGTGCAGCTCAAC CCGAGAGCCTTCAAGGCTATACAAG 286 60 32 U57439
AR CCCATCGACTATTACTTCCCACC TTCTCCTTCTTCCTGTAGTTTGA 291 55 35 M20133
AhR GGGATCGATTTCGAAGACATCAG AACGCCTGGGAGCCTGGAATCTC 789 60 30 U09000
ARNT GCTATAGTCATTCCCAGGTTTCT CATTGTTGTAGGTGTTGCT 765 60 34 U61184
LH{alpha} TCATGCTGTCCATGTTCCTGC TGACTGAAGCGTGTCAGAAGTCTG 427 62 24 V01253
LHß AGAGAATGAGTTCTGCCCAGTCTG AGGTCATTGGTTGAGTCCTGGG 275 65 27 J00479
LHR AGAGTGATTCCCTGGAAAGGA TCATCCCTTGGAAAGCATTC 273 56 33 M26199
GnRH GGCAAGGAGGAGGATCAAAT GCCCAGCTTCCTCTTCAATC 121 60 32 M31670
SF-1 ATTCGTACGACGAGGACCTG CTTGTACATGGGCCCAAACT 293 60 28 NM053344
DAX-1 AGGGTGGCATCCTCTACTCTATGC TCCTTGAGTGCAATCGGACG 529 54 34 X99470
GATA-4 AAGACACCAGCAGGTCCTCCTG CGCGGTGATTATGTCCCCTAG 339 58 36 NM144730
ß-Actin CACCATGTACCCAGGCATCGC AGGGGCCGGACTCATCGTAC 194 58 245 V01217

Except for the analyses described below, all PCRs were carried out under the conditions shown.

1 Accession number in GenBank is shown.

2 Cycle number for fetal liver CYP1A1 mRNA was 27.

3 Cycle number for the mRNA in cultured fetal testis and adult testis were 24 and 28, respectively.

4 Cycle number for testicular CYP11A1 mRNA in adults was 27.

5 In the analysis of ß-actin mRNA in adult testis and fetal brain, cycle numbers of 22 and 19 were used.