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Endocrinology, Vol 100, 988-993, Copyright © 1977 by Endocrine Society

ARTICLES

Appearances of responses to glucagon in cultured neoatal rat heart cells

CM Clark Jr, DO Allen and JF Clark

Heart cells from neonatal rats have been cultured. The ability of 10(- 5)M glucagon to stimulate adenylyl cyclase activity, and to increase the cAMP concentration and the beating rate in these cells was followed as a function of time in culture. The cultured cells show no response to 10(-5)M glucagon until 5 weeks. By contrast, the cells do respond to 10(-5)M epinephrine with an increase in beat rate, adenylyl cyclase activity and cAMP levels when freshly prepared or after 1 week in culture. Previous studies on the newborn rat heart, acutely isolated, have also shown that the neonatal rat heart is insensitive to glucagon until 4-5 weeks after birth. We conclude that the cultured neonatal rat heart cells can also mature in the same time frame with respect to a glucagon response.




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Copyright © 1977 by The Endocrine Society