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Production of Antisera Against Electrophoretically Separated Relaxin and Immunofluorescent Localization of Relaxin in the Porcine Corpus Luteum

Department of Anatomy, University of Florida College of Medicine Gainesville, Florida 32610

¹ Reprint requests to: Dr. Lynn H. Larkin, Department of Anatomy, University of Florida College of Medicine, Gainesville, Florida 32610.

Abstract

Antisera to porcine relaxin were produced in rabbits injected with different fractions that had been separated by polyacrylamide gel electrophoresis (PAGE). Analyses by agar double immunodiffusion demonstrated that the different fractions of relaxin separated by PAGE have similar antigenic sites and the individual fractions are indistinguishable from one another by this procedure. Antiserum to porcine relaxin inhibited the interpubic ligament forming ability of the hormone in vivo. Indirect fluorescent antibody studies demonstrated that the hormone was localized only in the corpus luteum of the pregnant sow ovary. Large ovoid or polyhedral cells, assumed to be granulosa lutein cells, exhibited the heaviest fluorescence.

Footnotes

Supported by USPHS grant HD-8522.

² Current address: Dr. R. M. Oliver, Department of Anatomy, Eastern Virginia Medical School, 358 Mowbray Arch, Norfolk, Virginia 23507.

Received August 11, 1976.