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Endocrinology, doi:10.1210/endo-101-3-849
Endocrinology Vol. 101, No. 3 849-857
Copyright © 1977 by the Endocrine Society.
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Estrogen Induction of Plasma Vitellogenin in the Cockerel: Studies with a Phosvitin Antibody

RICHARD L. JACKSON, HU-YU LIN, J. T. SIMON MAO, LAWRENCE CHAN and ANTHONY R. MEANS

Departments of Cell Biology and Medicine, Baylor College of Medicine and The Methodist Hospital Houston, Texas 77030

Abstract

The effects of estrogen on plasma vitellogenin have been studied in the cockerel by immunoprecipitation techniques using an antiserum prepared against the egg yolk phosphoprotein, phosvitin. The antiserum gave precipitin lines of complete identity to phosvitin and to vitellogenin which was isolated from hen plasma by DEAEcellulose chromatography and by affinity chromatography using anti-phosvitin coupled to Sepharose 4B. The cross-reactivity of vitellogenin and phosvitin adds support to the concept that plasma vitellogenin is the precursor phosphoprotein of egg yolk phosvitin. In the three-week old cockerel, antiphosvitin produced no detectable immunoprecipitate in the plasma. However, after a single sc injection of diethylstilbestrol (2.5 mg), plasma vitellogenin levels began to increase at 4 h and reached a maximum 20–30 h after hormone administration. The increase in plasma levels of triglyceride paralleled those of vitellogenin. These studies suggest that there is no significant time lag in the estrogenic induction of plasma vitellogenesis in the cockerel; the longer lag periods observed by other investigators may be a function of the sensitivity of the assays used for detecting vitellogenin.

Footnotes

Supported by Health, Education and Welfare Research grant HL 16512-02 and Grant-in-Aid 75-914 of the American Heart Association.

Received January 3, 1977.






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Copyright © 1977 by The Endocrine Society