Endocrinology, Vol 101, 969-974, Copyright © 1977 by Endocrine Society

ARTICLES

In vivo evidence for the intermediary role of 3',5'-cyclic AMP in parathyroid hormone-induced stimulation of 1alpha,25-dihydroxyvitamin D3 synthesis in rats

N Horiuchi, T Suda, H Takahashi, E Shimazawa and E Ogata

Teh stimulatory effect of parathyroid hormone (PTH) on renal 1alpha- hydroxylation of 25-hydroxyvitamin D3 (25-OH-D3) was studied in thyro- parathyroidectomized (TPTX), vitamin D-deficient rats into which bovine PTH, theophylline, cAMP or dibutyryl cAMP (dbcAMP) was constantly infused. The accumulation in plasma of 1alpha,25-dihydroxy-vitamin D3 [1alpha,25-(OH)2-D3], produced from 25-OH-D3, was enhanced by infusion of either cAMP (0.9 MUMOL/H) OR DBCAMP (1 mumol/h) to a level similar to the maximum obtained by PTH (i95--7.5 U/h) infusion. A submaximal dose (1 U/h) of PTH caused a similar extent of stimulation, when infused with theophylline. When either 2 mumol/h of cAMP or 7.5 U/h of bovine PTH was infused starting 18 h after TPTX, the accumulation of 1alpha,25-(OH)2-D3 in plasma was similarly restored withing 6 h to the level found in the sham-operated animals. These results strongly support the concept that cAMP plays an important intermediary role in the stimulation of 1alpha,25-(OH)2-D3 production induced by both exogenous and endogenous PTH.

This article has been cited by other articles:

				A. Maiti, N. C. Hait, and M. J. Beckman Extracellular Calcium-sensing Receptor Activation Induces Vitamin D Receptor Levels in Proximal Kidney HK-2G Cells by a Mechanism That Requires Phosphorylation of p38{alpha} MAPK J. Biol. Chem., January 4, 2008; 283(1): 175 - 183. [Abstract] [Full Text] [PDF]

				C. Galli, L. A. Zella, J. A. Fretz, Q. Fu, J. W. Pike, R. S. Weinstein, S. C. Manolagas, and C. A. O'Brien Targeted Deletion of a Distant Transcriptional Enhancer of the Receptor Activator of Nuclear Factor-{kappa}B Ligand Gene Reduces Bone Remodeling and Increases Bone Mass Endocrinology, January 1, 2008; 149(1): 146 - 153. [Abstract] [Full Text] [PDF]

				C. A. O'Brien, R. L. Jilka, Q. Fu, S. Stewart, R. S. Weinstein, and S. C. Manolagas IL-6 is not required for parathyroid hormone stimulation of RANKL expression, osteoclast formation, and bone loss in mice Am J Physiol Endocrinol Metab, November 1, 2005; 289(5): E784 - E793. [Abstract] [Full Text] [PDF]

				A. Matsunuma, T. Kawane, T. Maeda, S. Hamada, and N. Horiuchi Leptin Corrects Increased Gene Expression of Renal 25-Hydroxyvitamin D3-1{alpha}-Hydroxylase and -24-Hydroxylase in Leptin-Deficient, ob/ob Mice Endocrinology, March 1, 2004; 145(3): 1367 - 1375. [Abstract] [Full Text] [PDF]

				N. YOSHIDA, T. YOSHIDA, A. NAKAMURA, T. MONKAWA, M. HAYASHI, and T. SARUTA Calcitonin Induces 25-Hydroxyvitamin D3 1{alpha}-Hydroxylase mRNA Expression via Protein Kinase C Pathway in LLC-PK1 Cells J. Am. Soc. Nephrol., December 1, 1999; 10(12): 2474 - 2479. [Abstract] [Full Text]

				T. Shinki, Y. Ueno, H. F. DeLuca, and T. Suda Calcitonin is a major regulator for the expression of renal 25-hydroxyvitamin D3-1alpha -hydroxylase gene in normocalcemic rats PNAS, July 6, 1999; 96(14): 8253 - 8258. [Abstract] [Full Text] [PDF]

				T. YOSHIDA, N. YOSHIDA, A. NAKAMURA, T. MONKAWA, M. HAYASHI, and T. SARUTA Cloning of Porcine 25-Hydroxyvitamin D3 1{alpha}-Hydroxylase and Its Regulation by cAMP in LLC-PK1 Cells J. Am. Soc. Nephrol., May 1, 1999; 10(5): 963 - 970. [Abstract] [Full Text]

				G. JONES, S. A. STRUGNELL, and H. F. DeLUCA Current Understanding of the Molecular Actions of Vitamin D Physiol Rev, October 1, 1998; 78(4): 1193 - 1231. [Abstract] [Full Text] [PDF]

				H. L. Brenza, C. Kimmel-Jehan, F. Jehan, T. Shinki, S. Wakino, H. Anazawa, T. Suda, and H. F. DeLuca Parathyroid hormone activation of the 25-hydroxyvitamin D3-1alpha -hydroxylase gene promoter PNAS, February 17, 1998; 95(4): 1387 - 1391. [Abstract] [Full Text] [PDF]

				T. Shinki, H. Shimada, S. Wakino, H. Anazawa, M. Hayashi, T. Saruta, H. F. DeLuca, and T. Suda Cloning and expression of rat 25-hydroxyvitamin D3-1alpha -hydroxylase cDNA PNAS, November 25, 1997; 94(24): 12920 - 12925. [Abstract] [Full Text] [PDF]

				N Horiuchi, M. Holick, J. Potts Jr, and M Rosenblatt A parathyroid hormone inhibitor in vivo: design and biological evaluation of a hormone analog Science, June 3, 1983; 220(4601): 1053 - 1055. [Abstract] [PDF]