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Endocrinology, Vol 102, 330-333, Copyright © 1978 by Endocrine Society

ARTICLES

Methylation of estradiol-17 beta by a partially purified preparation of bovine pineal hydroxy-indole-O-methyltransferase

J Weisz, LV O'Brien and T Lloyd

A partially purified preparation of hydroxy-indole-O-methyltransferase (HIOMT) from bovine pineal was shown to O-methylate estradiol-17beta (E2). The HIOMT preparation was incubated with E2 and [3H]-S- adenosylmethionine (SAM). The [3H]-3 methyl-ether or estradiol [MeO-E2] produced was identified by thin layer chromatography (TLC) and crystallization to constant 3H/14C ratio in the presence of [14C]-MeO- E2 and unlabeled MeO-E2 standards. The kinetics of the enzyme reaction for melatonin formation from N-acetyl-serotonin (NAS) and for MeO-E2 were compared.




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Copyright © 1978 by The Endocrine Society