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Vitamin D and Cartilage. II. Biological Activity of 25-Hydroxycholecalciferol and 24,25- and 1,25-Dihydroxycholecalciferols on Cultured Growth Plate Chondrocytes^*

M. T. CORVOL, M. F. DUMONTIER, M. GARABEDIAN and R. RAPPAPORT

Unité de Recherche sur les Maladies du Rein et du Métabolisme chez l’Enfant, INSERM U-30 and Laboratoire des Tissus Calcifiés CNRS ER 126, Hópital des Enfants Malades Paris, France

Abstract

In order to study the effect of vitamin D₃, and some of its metabolites on cartilage, chondrocytes from the proliferative zone of rabbit growth plate cartilage were isolated and grown in culture. The ³⁵SO₄ incorporation into proteoglycans extracted from the cells or into macromolecules obtained after dialysis of the culture medium was evaluated in the presence of vitamin D₃, synthetic 25-hydroxycholecalciferol (25-(0H)D₃), 24,25- and 1,25-dihydroxycholecalciferols (24,25-(OH)₂D₃ and 1,25-(OH)₂D₃). At concentrations between 2.4 X 10^-l3 M and 2.4 x 10^-10 M, 24,25-(OH)₂D₃) was stimulatory. l,25-(OH)₂D₃ was also active at 2.4 X 10^-11 M and 1.2 X 10^-10 M concentrations. Higher concentrations of 25-(OH)₂D₃) (2.5 x 10^-8 M) were necessary to obtain a similar response. In contrast, vitamin D₃ was inactive at concentrations ranging from 10_-14 M-10_-7 M. A polar derivative was obtained after incubation of chondrocytes or cartilage tissue with 25-(OH)₂D₃, and was presumed to be 24,25-(OH)₂D₃ This cartilage polar derivative was found to be equipotent to both synthetic and biosynthetic 24,25-(OH)₂D₃ in the cultured chondrocytes bioassay system. (Endocrinology 102: 1269, 1978)

Footnotes

^* This study was supported by grants from Delegation Generate a la Recherche Scientifique et Technique no. 76-7-07-06. Part of this study was reported at the 1976 Second International Workshop on Calcified Tissue, Israel.

To whom requests for reprints should be addressed at: Hopital des Enfants Malades, Tour Technique 6 erne etage, 149, rue de Sevres, 75015 Paris, France.

Received February 28, 1977.

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