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FORMATION OF DES-ASP-ANGIOTENSIN II IS NOT AN OBLIGATORY STEP IN THE STEROIDOGENIC ACTION OF ANGIOTENSIN II IN THE CANINE ADRENAL

Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health Bethesda, Maryland 20014

Abstract

A comparison of the receptor binding properties and jLn vitro metabolism of angiotensin II and [des-Asp¹]-angiotensin II was performed in collagenase-dispersed glomerulosa cells from dog adrenal glands. Each peptide was bound rapidly and reversibly to isolated glomerulosa cells, with similar equilibrium and kinetic constants of association and dissociation. The labeled peptide that was bound to glomerulosa cells after incubation with ¹²⁵I- and ³H-angiotensin II at 37C for 30-60 minutes was predominantly angiotensin II, with about 15% [des-Asp¹]-angiotensin II. These findings demonstrate that local formation of the heptapeptide is not an obligatory step in the action of angiotensin II on aldosterone production in the canine adrenals.

Footnotes

¹ Present address: Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106

Received November 15, 1977.

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