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Down Regulation of Insulin Receptors in Primary Cultures of Adult Rat Hepatocytes in Monolayer^*

WILLIAM G. BLACKARD, PHILIP S. GUZELIAN and MARY E. SMALL

Department of Medicine, Medical College of Virginia Richmond, Virginia 23298

Address requests for reprints to: Dr. William G. Blackard, P.O. Box 155, MCV Station, Richmond, Virginia 23298.

Abstract

Down regulation of insulin receptor number by insulin at physiological concentrations (10^-9 M) has been demonstrated in primary cultures of adult rat liver parenchymal cells in monolayer. However, because insulin is rapidly degraded in this culture system, special efforts are necessary to maintain the concentration of insulin in the medium in order to evoke the down regulation phenomenon.

Footnotes

^* These investigations were supported by Grants AM-18903 and AM-18976 from the NIH.

Recipient of USPHS Clinical Investigator Award in Gastroenterology 5R08-AM-00128.

Received September 14, 1977.

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