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Lack of Effect of Noradrenergic Denervation of the Hypothalamus and Medial Preoptic Area on the Feedback Regulation of Gonadotropin Secretion and the Estrous Cycle of the Rat^*

Biological Sciences Research Center, Departments of Pharmacology, Medicine, and the Neurobiology Program, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27514

Address all correspondence and requests for reprints to: Dr. John S. Kizer, Biological Sciences Research Center of the Child Development Research Institute, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514.

Abstract

In order to determine the effect of noradrenergic denervation of the hypothalamus and preoptic area on the regulation of gonadotropin secretion in the rat, bilateral injections of 6-OH-dopamine (8 µg free base in 2 µl) or vehicle were made in caudal, ventral pons. After the placement of such lesions, concentrations of norepinephrine were markedly reduced throughout the hypothalamus and preoptic area (median eminence, 60–76%; arcuate nucleus, 75–80%; dorsomedial nucleus, 65–81%; suprachiasmatic nucleus, 70%; periventricular nucleus, 85%; paraventricular nucleus, 74%; ventromedial nucleus, 82%; and medial preoptic area, 82%), whereas concentrations of dopamine in these same areas were unchanged. Despite the placement of such lesions, serum LH and FSH levels in both intact and castrated rats were not significantly different from FSH and LH values in nonlesioned controls (intact and castrated). In addition, all lesioned female animals reinitiated consecutive 4-day estrous cycles, with the mean number of days between surgery and reinitiation of estrous being identical to that of controls. From these findings, two conclusions seem warranted: 1) nuclei within the hypothalamus and preoptic area receive the vast majority of their noradrenergic innervation by the ventral norepinephrine bundle, and 2) normal regulation of LH and FSH secretion in castrated and intact males as well as in normally cycling females is possible despite a substantial denervation of the noradrenergic innervation of the hypothalamus and medial preoptic area.

Footnotes

^* This work was supported by a training grant from NIGMS (5-T32-GM-07040), USPHS Neurobiology Postdoctoral Fellowship MH-14277, NIH Fellowship 5-F32-NS-05058, NIMH Grant NIMH-L-Rol-MH-28899, and NICHHD Grant 5-P30-HD03110.

Received October 12, 1977.

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