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Endocrinology, Vol 103, 644-647, Copyright © 1978 by Endocrine Society

ARTICLES

Inhibition of de novo synthesis of FSH by the Sertoli cell factor (SCF)

M Chowdhury, A Steinberger and E Steinberger

The effects of SCF, a substance produced by isolated rat Sertoli cells in culture, on the synthesis of FSH and LH were studied in vitro. Anterior pituitaries from adult male rats were organ-cultured 24-48h in the presence and absence of SCF in the culture medium. The incorporation of 3H-leucine into FSH and LH was determined following 6h incubation with this labelled precursor and immunoprecipitation with specific FSH or LH antibodies. Total FSH and LH were measured by radioimmunoassay. The results indicate that SCF can selectively reduce the incorporation of 3H-leucine into immunoprecipitable FSH without decreasing the incorporation into LH.


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G. V. Childs, B. T. Miller, and W. L. Miller
Differential Effects of Inhibin on Gonadotropin Stores and Gonadotropin-Releasing Hormone Binding to Pituitary Cells from Cycling Female Rats
Endocrinology, April 1, 1997; 138(4): 1577 - 1584.
[Abstract] [Full Text] [PDF]


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