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Evidence for the Involvement of Guanosine 3',5'-Cyclic Moiiophosphate in the Regulation of Gonadotropin Release^*

Department of Physiology, The University of Texas Health Science Center at Dallas, Southwestern Medical School Dallas, Texas 7523

Address requests for reprints to: Dr. S. M. McCann, University of Texas, Southwestern Medical School, Department of Physiology, 5323 Harry Hines Boulevard, Dallas, Texas 75235.

Abstract

Addition of dibutyryl adenosine 3',5'- cyclic monophosphate (DBcAMP) or dibutyryl guanosine 3',5'-cyclic monophosphate (DBcGMP; 1–6 mM) to enzymatically dispersed, overnight-cultured rat anterior pituitary cell preparations stimulated the release of gonadotropins (LH and FSH) from the cells into the incubation medium. Stimulation of gonadotropin release by DBcGMP was observed after only 10 min of incubation, whereas that caused by DBcAMP appeared at 180 min. Synthetic LHRH (2.5 x 10^-9 M) induced a small, transient increase in intracellular cAMP content (+16%, P < 0.05, after 5 min coincubation), while levels of cGMP in the same cells were rapidly and markedly low for 2 h. The decrease in cGMP content was accompanied by a discharge of gonadotropins lasting for 2 h, which was detectable after 5 min in the case of LH and after 10 min in the case of FSH. These results strongly suggest that cGMP might be an intracellular mediator in the process of LHRH-stimulated release of gonadotropins.

Footnotes

^* This work was supported by grants from NIH (AM- 10073 and HD-05151), the Ford Foundation, and the Texas Population Research Institute.

Received November 11, 1976.

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