This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by PERROT, M. Articles by MILGROM, E. Search for Related Content PubMed PubMed Citation Articles by PERROT, M. Articles by MILGROM, E.

Immunochemical Studies of Guinea Pig Progesterone- Binding Plasma Protein^*

Groupe de Recherches sur la Biochimie Endocrinienne et la Reproduction (INSERM U-135), Faculté de Médecine Paris Sud 94270 Bicêtre, France

Address reprint requests to: Dr. Edwin Milgrom, Hopital de Bicetre, 78 rue du General Leclerc, 94270 Kremlin- Becetre, France.

Abstract

An antiserum specific for guinea pig progesterone- binding plasma protein (PBP) has been prepared. Using a very sensitive immunoenzymatic assay, PBP could be detected not only in pregnant guinea pig plasma (970 µg/ml of plasma at day 40–60 of pregnancy), but also in the plasma of fetuses (2.77 µg/ml), umbilical arteries (1.79 µg/ml), umbilical vein (2.90 µg/ml), and in amniotic fluid (0.47 µg/ml). The protein was also found in low concentration in the plasma of nonpregnant females (2.10 µg/ml) and males (1.56 µg/ml).

The antiserum was used to search for immunological similarities between various steroid-binding proteins. No cross reaction was found with cavian or human corticosteroid-binding globulin and human sex steroidbinding plasma protein. There was no cross reaction between guinea pig PBP and PBP of other pregnant hystricomorphs (viscacha, degu, and coypu). Moreover, no evidence was found of an interaction between guinea pig uterine progesterone receptor and the anti-PBP antiserum.

Footnotes

^* This work was supported by INSERM and CNRS.

Received January 4, 1978.

This article has been cited by other articles:

				J. D. Graham and C. L. Clarke Physiological Action of Progesterone in Target Tissues Endocr. Rev., August 1, 1997; 18(4): 502 - 519. [Abstract] [Full Text] [PDF]