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Cytoplasmic 17β-[³H]Estradiol Binding in Rat Adipose Tissues^*

GEORGE N. WADE and JANET M. GRAY

Department of Psychology, University of Massachusetts Amherst, Massachusetts 01003

Abstract

Ovarian hormones, particularly 17β-estradiol, have important effects on body fat levels in rats, but it is not known whether 17β-estradiol can act directly on various fat depots to affect adiposity or whether these effects are entirely indirect (e.g. via food intake, exercise, or various metabolic actions). We have found high affinity, estrogen-specific macromolecular binding of 17β-[³H]estradiol in the cytoplasmic fraction of adipose tissues from ovariectomized rats. Saturation analysis indicates a K_d of 7.4 x 10^-10 M, and binding is inhibited by unlabeled 17β-estradiol or 11β-methoxy-17- ethynyl-l,3,5(10)-triene-3,17β-diol (R2858) but not by progesterone, 5-dihydrotestosterone, or cortisol. 17β- [³H]Estradiol binding is virtually abolished by incubation with pronase but not with DNase or RNase, indicating that the binding macromolecule is probably a protein. Binding is seen in all adipose tissues studied, including abdominal, sc, and brown fat. Binding site concentration is highest in parametrial fat pads, followed by retroperitoneal, brown, omental, and inguinal depots. Binding is also seen in the cytoplasmic fraction of isolated parametrial adipocytes.

These data indicate that the various adipose tissues might be estrogen target tissues in rats. Therefore, it is possible that estrogenic effects on body weight and composition could be mediated in part by direct estrogen action on adipose tissues.

Footnotes

^* This work was supported by NINCDS Research Grant NS-10873, NINCDS Research Career DevelopmentAward NS-00090, and NIAMDD Research GrantAM-20785

To whom requests for reprints should be addressed

Received January 11, 1978.

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