This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by LINDENBERG, J. A. Articles by BALLARD, P. L. Search for Related Content PubMed PubMed Citation Articles by LINDENBERG, J. A. Articles by BALLARD, P. L.

Triiodothyronine Nuclear Binding in Fetal and Adult Rabbit Lung and Cultured Lung Cells^*

JEFFREY A. LINDENBERG, ARLETTE BREHIER and PHILIP L. BALLARD

Cardiovascular Research Institute and Department of Pediatrics, University of California San Francisco, California 94143

Address all correspondence and requests for reprints to: P. L. Ballard, M.D., Cardiovascular Research Institute, University of California, San Francisco, California 94143; phone, (415)666-1940.

Abstract

To investigate the possible mechanism of thyroid hormone action in the lung, we examined fetal and adult rabbit lung, and cell lines derived from lung, for specific nuclear binding sites for T₃. Using incubation of isolated nuclei with L-[¹²⁵I]T₃ at 37 C, we found approximately 2400 specific binding sites/cell in fetal lung and 1120 sites/cell in adult lung, with a similar dissociation constant (500 pM) for both tissues. The L- 2 and A549 cell lines, which may have originated from pulmonary type II alveolar cells, contained 2280 and 1580 nuclear sites/cell, and the dissociation constants were 280 and 200 pM, respectively. In fetal lung, the ability of various analogs to compete for L-T₃ (100%) binding was: 3,5-diiodo-3'-isopropylthyronine, 81%; DT₃, 73%; L-T₄, 6.7%; 3,3'-diiodothyronine, 0.19%; 3,5-dimethyl- 3'-isopropyl-L-thyronine, 0.15%; and rT₃, 0.08%.

These findings indicate that both fetal and adult lung, and cultured lung cells, contain specific nuclear binding sites for T₃, suggesting that these tissues and their type II alveolar cells may be directly influenced by thyroid hormones.

Footnotes

^* This work was supported by Pulmonary Specialized Centers of Research Grant HL-19185 from the NHLBI.

James Scholar medical student at the University of Illinois, Chicago, Illinois.

Received January 20, 1978.

This article has been cited by other articles:

				K. Archavachotikul, T. J. Ciccone, M. R. Chinoy, H. C. Nielsen, and M. V. Volpe Pre- and Postnatal Lung Development, Maturation, and Plasticity: Thyroid hormone affects embryonic mouse lung branching morphogenesis and cellular differentiation Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L359 - L369. [Abstract] [Full Text] [PDF]