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Endocrinology, Vol 104, 157-161, Copyright © 1979 by Endocrine Society

ARTICLES

Stimulation of thyroglobulin synthesis in thyroid polysomes by soluble factors from reticulocyte lysate

GN Burrow and MS Jansen

The addition of rabbit reticulocyte lysate supernate to membrane-bound calf thyroid polysomes resulted in stimulation of [3H]leucine incorporation into acid-precipitable material; 91% of these total radioactive peptides was precipitated by thyroglobulin antibodies. The ability of reticulocyte lysate supernate to stimulate thyroglobulin syntheis was pH and temperature sensitive. The addition of 25 microM aurintricarboxylic acid resulted in an 85% inhibition of [3H]leucine incorporation.




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Copyright © 1979 by The Endocrine Society