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Endocrinology, Vol 105, 774-777, Copyright © 1979 by Endocrine Society

ARTICLES

Dexamethasone effect on the phospholipid content of isolated fat cell ghosts from adrenalectomized rats

DK Murray, A Ruhmann-Wennhold and DH Nelson

The lipid composition of sonicated ghosts from isolated fat cells has been investigated. Epididymal fat cells from adrenalectomized rats were incubated with and without 8 x 10(-8) M dexamethasone for 3 h. Phospholipids and cholesterol were isolated from membrane ghosts prepared from these cells. There was a 61% increase in membrane sphingomyelin from cells incubated with dexamethasone (P less than 0.025). No significant change was observed in other phospholipids, cholesterol, or fatty acid components of the phospholipids.


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