Endocrinology, Vol 107, 1065-1068, Copyright © 1980 by Endocrine Society

ARTICLES

Acetylcholine stimulates insulin, glucagon, and somatostatin release in the perfused chicken pancreas

RN Honey and GC Weir

To evaluate the possible cholinergic control of islet hormone secretion, acetylcholine (10 microM) was perfused into the isolated chicken pancreas-duodenum, with perfusate concentrations of 2.8, 14, or 42 mM glucose. Prompt and significant stimulation of insulin, glucagon, and somatostatin secretion was seen when the glucose concentration was 14 mM, and this stimulation was blocked by preinfusion of atropine sulfate (50 microM). Similar stimulation of all three peptides was seen when the perfusate concentration of glucose was 42 mM. When the perfusate glucose concentration was 2.8 mM, the insulin response to acetylcholine was absent and the response by glucagon could not be discerned, perhaps because release was close to maximal. The somatostatin response, however, was only slightly diminished from that seen at higher glucose levels. The B cell response to acetycholine in the chicken was considerably greater than the weak responses seen to high glucose or arginine, suggesting a dominant role for cholinergic control of avian insulin secretion. In addition, the parasympathetic nervous system may also play a role in the secretory control of A- and D cells.

This article has been cited by other articles:

				A. Duttaroy, C. L. Zimliki, D. Gautam, Y. Cui, D. Mears, and J. Wess Muscarinic Stimulation of Pancreatic Insulin and Glucagon Release Is Abolished in M3 Muscarinic Acetylcholine Receptor-Deficient Mice Diabetes, July 1, 2004; 53(7): 1714 - 1720. [Abstract] [Full Text] [PDF]

				P. Gilon and J.-C. Henquin Mechanisms and Physiological Significance of the Cholinergic Control of Pancreatic {beta}-Cell Function Endocr. Rev., October 1, 2001; 22(5): 565 - 604. [Abstract] [Full Text] [PDF]