This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by INOUE, K. Articles by KONDO, Y. Search for Related Content PubMed PubMed Citation Articles by INOUE, K. Articles by KONDO, Y.

Effect of Thyrotropin on Cell Orientation and Follicle Reconstruction in Rotated Suspension Culture of Hog Thyroid Cells^*

Departments of Morphology (K.I.), Pharmaceutical Chemistry (R.H.), and Department of Physical Biochemistry (Y.K.), Institute of Endocrinology, Gunma University Maebashi, 371 Japan

Address requests for reprints to: Dr. Kinji Inoue, Department of Morphology, Institute of Endocrinology, Gunma University, Maebashi, 371 Japan.

Abstract

Isolated porcine thyroid cells were cultured in suspension by rotation on a gyratory shaker. In the absence of TSH, the cells associated into small, globular, and hollow aggregates or pseudofollicles and had microvilli only at the outer surface of the pseudofollicles which faced the culture medium. These pseudofollicles were inactive in terms of thyroglobulin synthesis and iodination. In the presence of TSH, cell aggregates were enlarged to 1 mm in diameter. Inside the aggregates, the cells were reorganized into numerous functional follicles in which microvilli were oriented towards the follicular lumen. In both the presence and absence of TSH, Golgi apparatuses were found in the cytoplasm near the cell periphery bearing microvilli. These findings indicate that the TSH effects on thyroid cell morphology are to arrange the polarity of follicular cells in a proper direction and to enlarge the aggregates they form.

Footnotes

^* This work was supported by a grant from the Ministry of Education, Science, and Culture of Japan.

Received September 14, 1980.

This article has been cited by other articles:

				M. Murakami, O. Araki, Y. Hosoi, Y. Kamiya, T. Morimura, T. Ogiwara, H. Mizuma, and M. Mori Expression and Regulation of Type II Iodothyronine Deiodinase in Human Thyroid Gland Endocrinology, July 1, 2001; 142(7): 2961 - 2967. [Abstract] [Full Text] [PDF]

				F Andre, P Filippi, and H Feracci Merosin is synthesized by thyroid cells in primary culture irrespective of cellular organization J. Cell Sci., January 1, 1994; 107(1): 183 - 193. [Abstract] [PDF]