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Endocrinology, Vol 107, 2110-2112, Copyright © 1980 by Endocrine Society

ARTICLES

Immunocytochemical localization of relaxin in endometrial glands of the pregnant guinea pig

R Pardo, LH Larkin and PA Fields

Relaxin was localized in the cytoplasm of endometrial gland cells in the uterus of day 60 pregnant guinea pigs with the peroxidase- antiperoxidase method. The immunoprecipitate was located over the apical portions of the glandular cells. Stain was not found over non- endometrial portions of the uterus, endometrial surface epithelium or cells of the uterine cervical glands. Agar double-immunodiffusion analyses demonstrated a reaction of identity when extracts of uterus from day 60 pregnant guinea pigs and a porcine relaxin standard were tested with an antiserum produced against highly purified porcine relaxin. An extract of uteri from day 60 pregnant guinea pigs gave a positive response in the mouse uterus bioassay for relaxin.




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Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1980 by The Endocrine Society