Endocrinology, Vol 108, 213-219, Copyright © 1981 by Endocrine Society

ARTICLES

Functional properties of hormonally responsive cultured normal and malignant rat osteoblastic cells

NC Partridge, D Alcorn, VP Michelangeli, BE Kemp, GB Ryan and TJ Martin

Certain metabolic properties of hormonally responsive osteogenic sarcoma cells derived from a transplantable rat tumor have been compared with those of related normal rat bone cells. All studies were carried out on cells grown in monolayer culture. Normal rat bone cells derived by repeated collagenase/trypsin digestion of newborn rat calvaria. Bone cells selected for comparison were thought to be osteoblast-like, as judged by enrichment of alkaline phosphatase and adenylate cyclase responsiveness to parathyroid hormone and prostaglandin E2. The adenylate cyclases of the two cell strains were similarly stimulated by a range of prostanoids and their metabolites and analogs. Morphology showed the two cell strains to be similar; the only obvious difference was a multilayering of cells in the sarcoma cultures, while the normal cultures showed abundant extracellular fibril formation which was not seen in the tumor cells. Investigation of the cAMP-dependent protein kinase isoenzymes showed the presence of two forms in both cell types, one eluting at a low salt concentration and the other at a high salt concentration. There was approximately twice the amount of the first isoenzyme compared to the second isoenzyme. The results indicate the usefulness of the two cell strains to elucidate further the molecular mechanisms of action of parathyroid hormone and prostaglandins.

This article has been cited by other articles:

				S. Yu, R. T. Franceschi, M. Luo, X. Zhang, D. Jiang, Y. Lai, Y. Jiang, J. Zhang, and G. Xiao Parathyroid Hormone Increases Activating Transcription Factor 4 Expression and Activity in Osteoblasts: Requirement for Osteocalcin Gene Expression Endocrinology, April 1, 2008; 149(4): 1960 - 1968. [Abstract] [Full Text] [PDF]

				R.-M. CHEN, G.-J. WU, H.-C. CHANG, J.-T. CHEN, T.-F. CHEN, Y.-L. LIN, and T.-L. CHEN 2,6-Diisopropylphenol Protects Osteoblasts from Oxidative Stress-Induced Apoptosis through Suppression of Caspase-3 Activation Ann. N.Y. Acad. Sci., May 1, 2005; 1042(1): 448 - 459. [Abstract] [Full Text] [PDF]

				W.-P. HO, T.-L. CHEN, W.-T. CHIU, Y.-T. TAI, and R.-M. CHEN Nitric Oxide Induces Osteoblast Apoptosis through a Mitochondria-Dependent Pathway Ann. N.Y. Acad. Sci., May 1, 2005; 1042(1): 460 - 470. [Abstract] [Full Text] [PDF]

				D. Jiang, R. T. Franceschi, H. Boules, and G. Xiao Parathyroid Hormone Induction of the Osteocalcin Gene: REQUIREMENT FOR AN OSTEOBLAST-SPECIFIC ELEMENT 1 SEQUENCE IN THE PROMOTER AND INVOLVEMENT OF MULTIPLE SIGNALING PATHWAYS J. Biol. Chem., February 13, 2004; 279(7): 5329 - 5337. [Abstract] [Full Text] [PDF]

				M. M. Tabb, A. Sun, C. Zhou, F. Grun, J. Errandi, K. Romero, H. Pham, S. Inoue, S. Mallick, M. Lin, et al. Vitamin K2 Regulation of Bone Homeostasis Is Mediated by the Steroid and Xenobiotic Receptor SXR J. Biol. Chem., November 7, 2003; 278(45): 43919 - 43927. [Abstract] [Full Text] [PDF]

				L. Qin, P. Qiu, L. Wang, X. Li, J. T. Swarthout, P. Soteropoulos, P. Tolias, and N. C. Partridge Gene Expression Profiles and Transcription Factors Involved in Parathyroid Hormone Signaling in Osteoblasts Revealed by Microarray and Bioinformatics J. Biol. Chem., May 23, 2003; 278(22): 19723 - 19731. [Abstract] [Full Text] [PDF]

				T. Hirayama, L. Danks, A. Sabokbar, and N. A. Athanasou Osteoclast formation and activity in the pathogenesis of osteoporosis in rheumatoid arthritis Rheumatology, November 1, 2002; 41(11): 1232 - 1239. [Abstract] [Full Text] [PDF]

				R. R. Miles, J. P. Sluka, D. L. Halladay, R. F. Santerre, L. V. Hale, L. Bloem, K. Thirunavukkarasu, R. J. S. Galvin, J. M. Hock, and J. E. Onyia ADAMTS-1: A Cellular Disintegrin and Metalloprotease with Thrombospondin Motifs Is a Target for Parathyroid Hormone in Bone Endocrinology, December 1, 2000; 141(12): 4533 - 4542. [Abstract] [Full Text] [PDF]

				I Itonaga, A Sabokbar, D W Murray, and N A Athanasou Effect of osteoprotegerin and osteoprotegerin ligand on osteoclast formation by arthroplasty membrane derived macrophages Ann Rheum Dis, January 1, 2000; 59(1): 26 - 31. [Abstract] [Full Text]

				Y. Yoshiko, A. Son, S. Maeda, A. Igarashi, S. Takano, J. Hu, and N. Maeda Evidence for Stanniocalcin Gene Expression in Mammalian Bone Endocrinology, April 1, 1999; 140(4): 1869 - 1874. [Abstract] [Full Text]

				M. Weinreb, A. Grosskopf, and N. Shir The anabolic effect of PGE2 in rat bone marrow cultures is mediated via the EP4 receptor subtype Am J Physiol Endocrinol Metab, February 1, 1999; 276(2): E376 - E383. [Abstract] [Full Text] [PDF]

				C. M. Stanford, P. A. Jacobson, E. D. Eanes, L. A. Lembke, and R. J. Midura Rapidly Forming Apatitic Mineral in an Osteoblastic Cell Line (UMR 106-01 BSP) J. Biol. Chem., April 21, 1995; 270(16): 9420 - 9428. [Abstract] [Full Text] [PDF]