help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hudson, P.
Right arrow Articles by Coghlan, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hudson, P.
Right arrow Articles by Coghlan, J.

Endocrinology, Vol 108, 353-356, Copyright © 1981 by Endocrine Society

ARTICLES

Hybridization histochemistry: use of recombinant DNA as a "homing probe" for tissue localization of specific mRNA populations

P Hudson, J Penschow, J Shine, G Ryan, H Niall and J Coghlan

A procedure, termed hybridization histochemistry, has been developed to locate in specially prepared whole sections of tissue those areas which contain specific mRNA populations. Three 32P-labelled recombinant DNA probes were used; one complementary to endorphin mRNA, one complementary to growth hormone mRNA and one a fragment of bacterial DNA. The specific cell populations or tissue regions binding the probe were identified by autoradiography. Hybridization histochemistry is thus similar in principle to immunohistochemical procedures. The endorphin probe consistently labelled the rat pituitary pars intermedia which is known to be particularly rich in the corresponding mRNA. Likewise the growth hormone probe specifically labelled the anterior pituitary. Control tissues were not labelled by either probe, nor did the bacterial DNA probe significantly label any tissue, providing further evidence of the specificity of the procedure. These results, which are highly reproducible, indicate that the mRNA species for endorphin and growth hormone are present in whole sections of pituitary in a physical state which leaves them accessible to cDNA probes. This initial success provides encouragement that hybridization histochemistry, with further refinement, should have wide applicability in the localization and semi-quantitative analysis of intracellular mRNA in whole frozen sections of tissue.


This article has been cited by other articles:


Home page
 DevelopmentHome page
T Thomas and M Dziadek
Capacity to form choroid plexus-like cells in vitro is restricted to specific regions of the mouse neural ectoderm
Development, January 1, 1993; 117(1): 253 - 262.
[Abstract] [PDF]

Home page
 ScienceHome page
A. Mason, J. Hayflick, R. Zoeller, W. Young 3rd, H. Phillips, K Nikolics, and P. Seeburg
A deletion truncating the gonadotropin-releasing hormone gene is responsible for hypogonadism in the hpg mouse
Science, December 12, 1986; 234(4782): 1366 - 1371.
[Abstract] [PDF]

Home page
 ScienceHome page
T Hokfelt, O Johansson, and M Goldstein
Chemical anatomy of the brain
Science, September 21, 1984; 225(4668): 1326 - 1334.
[Abstract]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1981 by The Endocrine Society