help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Balsam, A.
Right arrow Articles by Ingbar, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Balsam, A.
Right arrow Articles by Ingbar, S. H.

Endocrinology, Vol 108, 472-477, Copyright © 1981 by Endocrine Society

ARTICLES

The influence of fasting and the thyroid state on the activity of thyroxine 5'-monodeiodinase in rat liver: a kinetic analysis of microsomal formation of triiodothyronine from thyroxine

A Balsam, F Sexton and SH Ingbar

In the present studies, we have evaluated the effects of fasting, variations in thyroid state, and interactions thereof on the kinetic properties of the microsomal enzyme in rat liver that generates T3 from T4. The formation of T3 and T4 (T3-neogenesis) in preparations of rat liver microsomes enriched with 10 mM dithiothreitol was monitored by assessing the formation of [125I]T3 from [125I]T4 in the presence of various concentrations of stable T4. T3-neogenesis in this system was a saturable process that obeyed the laws of first order enzyme kinetics. The effects of diverse in vivo manipulations on the kinetics of T3- neogenesis were assessed. The Vmax was markedly decreased in preparations from thyroidectomized animals, being, respectively, partially and completely restored to normal with daily maintenance therapy with T4, (0.5 and 1.5 microgram/100 g BW) and markedly increased after the administration of supraphysiological replacement doses of T4 (5.0 microgram/100 g . day). Km was not affected by alterations in thyroid state. In experiments concerning the effects of fasting, cognizance was taken of the fact that hypothyroidism regularly evolves as a consequence of fasting. The Vmax was decreased markedly, and Km was slightly decreased in preparations from intact rats fasted 72 h but not given physiological replacement with T4 or T3. In contrast, Vmax and Km were unchanged in hepatic microsomes from intact rats fasted 72 h but given daily parenteral replacement with T4 (1.5 microgram/100 g) or T3 (0.5 microgram/100 g). These data demonstrate that hypothyroidism resulting from either thyroidectomy or fasting produces decreased intrinsic activity of the microsomal T4 5'- monodeiodinase. In contrast, fasting without concurrent hypothyroidism does not influence the intrinsic activity of the hepatic T3-neogenetic enzyme. (Endocrinology 108: 472, 1981)




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1981 by The Endocrine Society