This Article Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints, Permissions and Rights Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by ANDREWS, W. W. Articles by OJEDA, S. R. Search for Related Content PubMed PubMed Citation Articles by ANDREWS, W. W. Articles by OJEDA, S. R.

A Quantitative Analysis of the Maturation of Steroid Negative Feedbacks Controlling Gonadotropin Release in the Female Rat: The Infantile-Juvenile Periods, Transition from an Androgenic to a Predominantly Estrogenic Control^*

W. W. ANDREWS and S. R. OJEDA

Department of Physiology, University of Texas Health Science Center at Dallas Dallas, Texas 75235

Address requests for reprints to: Dr. Sergio R. Ojeda, Department of Physiology, The University of Texas Health Science Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75235.

Abstract

To determine what ovarian or adrenal steroid (s) are involved in restraining gonadotropin secretion in infantile female rats, several experiments were performed. Adrenalectomy of 10-day-old rats markedly decreased serum progesterone (P) and, to a lesser extent, serum estradiol (E₂) levels. Androgen (A) levels were only transiently reduced, and serum dihydrotestosterone was not affected. Serum LH and FSH, on the other hand, increased only transiently after adrenalectomy. Ovariectomy (OVX) failed to decrease serum P and partially depressed serum E₂, but resulted in a marked fall of both testosterone (T) and dihydrotestosterone. Serum LH and FSH were increased in these animals. Replacement of pre-OVX serum T levels via Silastic capsules prevented the postcastration rise in both LH and FSH, suggesting that the increase in gonadotropins that follows OVX of 10-day-old rats is, to a large extent, a consequence of the loss of ovarian T. OVX of juvenile (27 days old) rats increased serum FSH and LH 48 h later and reduced both serum T and E₂ levels, with no significant change in serum P. Replacement of pre-OVX serum T levels depressed post-OVX serum FSH and LH titers only partially. In contrast, replacement of pre-OVX serum E₂ levels, effectively suppressed the postcastration rise in serum gonadotropins.

The results suggest that 1) during the infantile period, gonadotropin release is predominantly under ovarian androgenic inhibitory control, but this mechanism is not very effective, because basal serum gonadotropin levels, particularly FSH, are elevated; 2) the effectiveness of the A negative feedback on FSH remains unchanged during prepubertal development; and 3) the previously reported increase in E₂ negative feedback effectiveness that occurs after day 15 results in an E₂ inhibitory signal that surpasses that of As, so that during juvenile days, most of the ovarian steroidal inhibitory control on gonadotropin release is mediated by E₂.

Footnotes

^* This work was supported by NIH Grant HD-09988-4.

Predoctoral Fellow supported by NIH Training Grant HD-07062-1.

Received July 9, 1980.

This article has been cited by other articles:

				V. Matagne, C. Mastronardi, R. A. Shapiro, D. M. Dorsa, and S. R. Ojeda Hypothalamic Expression of Eap1 Is Not Directly Controlled by Ovarian Steroids Endocrinology, April 1, 2009; 150(4): 1870 - 1878. [Abstract] [Full Text] [PDF]

				S. Mazaud, C. J. Guigon, A. Lozach, N. Coudouel, M. G. Forest, H. Coffigny, and S. Magre Establishment of the Reproductive Function and Transient Fertility of Female Rats Lacking Primordial Follicle Stock after Fetal {gamma}-Irradiation Endocrinology, December 1, 2002; 143(12): 4775 - 4787. [Abstract] [Full Text] [PDF]

				M. C. H. Holland, S. Hassin, and Y. Zohar Effects of Long-Term Testosterone, Gonadotropin-Releasing Hormone Agonist, and Pimozide Treatments on Gonadotropin II Levels and Ovarian Development in Juvenile Female Striped Bass (Morone saxatilis) Biol Reprod, November 1, 1998; 59(5): 1153 - 1162. [Abstract] [Full Text]