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Endocrinology, Vol 108, 1810-1814, Copyright © 1981 by Endocrine Society

ARTICLES

Long term primary monolayer culture of adult murine magnocellular neurons

HS U, GF Erickson and WB Watkins

Primary monolayer culture of adult murine hypothalamic cells has been carried out for 2 months. Neuron-like cells as well as fibroblast, glial, and ependymal-like cells demonstrated characteristic morphological features which distinguished them from one another. In addition, immunocytochemical identification of cytoplasmic substances cross-reactive with neurophysin and (8-arginine)vasopressin further characterized specific magnocellular neuronal populations throughout the culture period. This culture system should provide a basis for studying neurosecretion at the cellular and molecular levels.




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Copyright © 1981 by The Endocrine Society