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Endocrinology, Vol 109, 1281-1283, Copyright © 1981 by Endocrine Society

ARTICLES

Photoaffinity labeling of luteinizing hormone releasing hormone receptor of rat pituitary membrane preparations

E Hazum

Specific LHRH receptor proteins of plasma membrane preparation from pituitary glands of the rat were identified using an 125I-labeled photoreactive LHRH derivative, [D-Lys6-N epsilon-azidobenzoyl]LHRH. This analog retained high binding affinity (apparent Kd value of 0.1 nM) to a single class of receptors. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated the specific photolabeling of two proteins, a major band with an apparent molecular weight of 60,000 daltons and a minor band of 48,000 daltons. The latter is probably a degradation product of the receptor.


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K. Eidne, C. Flanagan, and R. Millar
Gonadotropin-releasing hormone binding sites in human breast carcinoma
Science, September 6, 1985; 229(4717): 989 - 991.
[Abstract] [PDF]


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