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Endocrinology, Vol 109, 1473-1478, Copyright © 1981 by Endocrine Society

ARTICLES

Triiodothyronine augments the number of membrane-bound (Na+-K+)- adenosine triphosphatase units, but does not affect the sedimentation properties of plasma membrane components

CS Lo and TN Lo

We have previously demonstrated that T3 enhanced the de novo synthesis of renal cortical (Na+-K+)-dependent ATPase (NaK-ATPase) in the rat. A purified membrane fraction obtained from successive centrifugation of renal cortical crude homogenate was used in the above studies. To rule out a possible effect of T3 on plasma membrane properties, such as the sedimentation characteristic of NaK-ATPase, we have presently observed T3-dependent increases in the activity and number of NaK-ATPase units in a crude homogenate of rat renal cortex. The following results were obtained which substantiate a specific effect of T3 on the membrane- bound NaK-ATPase system. 1) Compared to the hypothyroid state, 43% and 44% increases in the activity and number of NaK-ATPase units, respectively, were observed in crude renal cortical homogenate of T3- treated hypothyroid rats. 2) In comparison with the crude homogenate prepared from hypothyroid rats, 4.8- and 113-fold increases in the specific activity of NaK-ATPase were obtained in the L and J fractions, respectively. Increases of similar relative magnitude in the L and J fractions were also shown in T3-treated hypothyroid and euthyroid rats. 3) No difference in the recovery of the number of NaK-ATPase units was observed from successive steps of the purification under different thyroid states. 4) Treatment of renal cortices from hypothyroid, T3- treated hypothyroid, and euthyroid rats with deoxycholate increased to the same extent NaK-ATPase activity and phosphorylated intermediate formation.


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