Endocrinology, Vol 110, 1465-1471, Copyright © 1982 by Endocrine Society

ARTICLES

Intracellular localization of prolactin receptor and prolactin in the rat ovary by immunocytochemistry

AE Dunaif, EA Zimmerman, HG Friesen and AG Frantz

The localization of the PRL receptor as well as of PRL has been studied by immunoperoxidase techniques in the ovaries of cycling, pregnant, and lactating rats. Specific antisera to the receptor and to the hormone were used. By light microscopy, immunostaining for the PRL receptor coincided with that for the hormone. Staining was found intracellularly in most components of the ovary, except the theca, and was most striking in the luteal cells. Both PRL and its receptor were concentrated heavily in the ovum. Beginning 24-36 h postpartum, there was a change in the pattern of luteal cell staining, with a shift in the intensity of staining products to the periphery of the luteal cell, giving a ring appearance to these cells. The results suggest roles for PRL in ovarian function involving both maintenance of the corpus luteum and maturation of the ovum. This study also demonstrates the intracellular localization of a polypeptide hormone in association with its specific receptor.

This article has been cited by other articles:

				R A Picazo, J P Garcia Ruiz, J Santiago Moreno, A Gonzalez de Bulnes, J Munoz, G Silvan, P L Lorenzo, and J C Illera Cellular localization and changes in expression of prolactin receptor isoforms in sheep ovary throughout the estrous cycle Reproduction, November 1, 2004; 128(5): 545 - 553. [Abstract] [Full Text] [PDF]

				C. R. McCudden, K. A. James, C. Hasilo, and G. F. Wagner Characterization of Mammalian Stanniocalcin Receptors. MITOCHONDRIAL TARGETING OF LIGAND AND RECEPTOR FOR REGULATION OF CELLULAR METABOLISM J. Biol. Chem., November 15, 2002; 277(47): 45249 - 45258. [Abstract] [Full Text] [PDF]

				M. Paciga, A. J. Watson, G. E. DiMattia, and G. F. Wagner Ovarian Stanniocalcin Is Structurally Unique in Mammals and Its Production and Release Are Regulated through the Luteinizing Hormone Receptor Endocrinology, October 1, 2002; 143(10): 3925 - 3934. [Abstract] [Full Text] [PDF]

				S. P. Tam, P. Lau, J. Djiane, D. J. Hilton, and M. J. Waters Tissue-Specific Induction of SOCS Gene Expression by PRL Endocrinology, November 1, 2001; 142(11): 5015 - 5026. [Abstract] [Full Text] [PDF]

				N. P. Vlahos, E. M. Bugg, M. J. Shamblott, J. Y. Phelps, J. D. Gearhart, and H. A. Zacur Prolactin receptor gene expression and immunolocalization of the prolactin receptor in human luteinized granulosa cells Mol. Hum. Reprod., November 1, 2001; 7(11): 1033 - 1038. [Abstract] [Full Text] [PDF]

				C. Bole-Feysot, V. Goffin, M. Edery, N. Binart, and P. A. Kelly Prolactin (PRL) and Its Receptor: Actions, Signal Transduction Pathways and Phenotypes Observed in PRL Receptor Knockout Mice Endocr. Rev., June 1, 1998; 19(3): 225 - 268. [Abstract] [Full Text]