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Localization of Anti-Müllerian Hormone in the Rough Endoplasmic Reticulum of the Developing Bovine Sertoli Cell Using Immunocytochemistry with a Monoclonal Antibody

Unité de Recherches de Génétique Médicate, INSERM U12, Hôpital des Enfants Malades, 75743 Paris Cedex 15, France

Address requests for reprints to: Dr. Dien Tran, Unité de Recherches de Génétique Médicale, INSERM U12, Hôpital des Enfants Malades, 149, rue de Sévres, 75743 Paris Cedex 15, France.

Abstract

Anti-Müllerian hormone (AMH) was localized in Sertoli cell cytoplasm of male fetal and neonatal calves by an immunocytochemical technique using a monoclonal antibody directed against bovine AMH. At the ultrastructural level, AMH accumulates in the cisternae of the rough endoplasmic reticulum, but is not detected in the Golgi apparatus or in secretion granules. In the fetus, the immunocytochemical reaction for AMH appears at 43 days postcoitum in differentiating seminiferous tubules, plateaus from 50 days postcoitum to 8 days post partum, and subsequently decreases to become barely detectable in the testis of the 3-month-old calf. This chronological evolution is in keeping with previous studies of testicular anti-Miillerian activity.

Received February 1, 1982.

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