Endocrinology, Vol 112, 104-112, Copyright © 1983 by Endocrine Society

ARTICLES

Subcellular localization of the receptor for gonadotropin-releasing hormone in pituitary and ovarian tissue

J Marian and PM Conn

The subcellular localization of GnRH receptors in weanling rat anterior pituitary and ovarian tissue was determined by radioligand binding in biochemically defined fractions prepared by differential and density centrifugation. Morphological identification of membrane organelles or fragments, visualized by electron microscopy, confirmed the biochemical characterization. The greatest amount of specific ligand binding in both tissues was measured in the crude membrane fraction, while small amounts were detected in other fractions. The distribution of pituitary binding sites in sucrose gradient subfractionation of crude membranes correlated with the distribution of plasma membrane (assessed by 5'- nucleotidase activity and electron microscopy), but not that of secretory granules (identified by immunoreactive LH) or lysosomes (acid phosphatase activity). These data suggest that the GnRH-binding sites detected by radioligand binding assay in pituitaries of rats not previously exposed to GnRH are localized almost exclusively in the plasma membrane fraction.

This article has been cited by other articles:

				K. R Sedgley, A. R Finch, C. J Caunt, and C. A McArdle Intracellular gonadotropin-releasing hormone receptors in breast cancer and gonadotrope lineage cells J. Endocrinol., December 1, 2006; 191(3): 625 - 636. [Abstract] [Full Text] [PDF]

				I. Y. Lebedeva, V. A. Lebedev, R. Grossmann, T. I. Kuzmina, and N. Parvizi Characterization of Growth Hormone Binding Sites in Granulosa and Theca Layers at Different Stages of Follicular Maturation and Ovulatory Cycle in the Domestic Hen Biol Reprod, October 1, 2004; 71(4): 1174 - 1181. [Abstract] [Full Text] [PDF]

				G. Halmos and A. V. Schally Changes in subcellular distribution of pituitary receptors for luteinizing hormone-releasing hormone (LH-RH) after treatment with the LH-RH antagonist cetrorelix PNAS, January 22, 2002; 99(2): 961 - 965. [Abstract] [Full Text] [PDF]

				A. Cornea, J. A. Janovick, X. Lin, and P. M. Conn Simultaneous and Independent Visualization of the Gonadotropin-Releasing Hormone Receptor and Its Ligand: Evidence for Independent Processing and Recycling in Living Cells Endocrinology, September 1, 1999; 140(9): 4272 - 4280. [Abstract] [Full Text]