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Endocrinology, Vol 112, 378-380, Copyright © 1983 by Endocrine Society

ARTICLES

Cells isolated from embryonic intestine synthesize 1,25- dihydroxyvitamin-D3 and 24,25-dihydroxyvitamin-D3 in culture

JE Puzas, RT Turner, GA Howard and DJ Baylink

Cells isolated from embryonic chick intestine in culture convert 25- hydroxyvitamin-D3 to a number of more polar metabolites. Two of these metabolites have been identified with chemical and chromatographic methods as 24,25-dihydroxyvitamin-D3 (24,25(OH)2D3) and 1,25- dihydroxyvitamin-D3 (1,25(OH)2D3). The enzymes conform to substrate saturation kinetics. The apparent Km for substrate for the synthesis of 1,25(OH)2D3 and 24,25(OH)2D3 is 70 nM and 29 nM, respectively.


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[Abstract] [Full Text] [PDF]


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