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A Homologous Radioreceptor Assay for Rat Follicle- Stimulating Hormone

Departments of Obstetrics and Gynecology Houston, Texas 77025
Departments of Obstetrics and Reproductive Medicine and Biology University of Texas Medical School at Houston Houston, Texas 77025

Address requests to reprints to: William Findley, Ph.D., Department of Obstetrics and Gynecology, Baylor College of Medicine, 1200 Moursund, Houston, Texas 77030.

Abstract

A homologous radioreceptor assay (RRA) for rat FSH (rFSH), which is both sensitive and easy to perform, is described. The receptor preparation is isolated from a 12,000 x G pellet of rat testes homogenate prepared with a high speed tissue grinder using Tris buffer. The sensitivity of the assay extends below 10 ng rFSH RP-1 (0.13 ng purified 1-3 rat FSH), and the range of the assay spans 2 orders of magnitude. The specific binding of the [¹²⁵I[rFSH tracer is 22-28% of the total tracer added. Such binding, which exceeds previously published values by 2- to 4-fold, allows the addition of relatively small amounts of total tracer radioactivity, which, in turn, contributes to a low nonspecific binding and highly reproducible values for replicates (coefficient of variation, 3.0%). This represents the first homologous RRA for rFSH using testicular receptors. Likewise, the sensitivity and reproducibility exceed those of previous RRAs for rFSH. {Endocrinology 113: 1081, 1983)

Received December 21, 1982.