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Endocrinology, Vol 113, 1427-1430, Copyright © 1983 by Endocrine Society

ARTICLES

Autoradiographic demonstration of high affinity nuclear binding and finite binding capacity of [3H]estradiol in mouse vaginal cells

GR Cunha, JM Shannon, KD Vanderslice, K McCormick and RM Bigsby

Vaginae from adult C57Bl/6J mice were analyzed for nuclear estrogen- binding sites by biochemical as well as in vitro steroid autoradiographic methods. Finite binding capacity (saturability) and high affinity binding were demonstrated in stromal cell nuclei (Kd = 1.0 nM) by autoradiographic methods and in nuclear extracts of vaginal homogenates (Kd = 1.9 nM) by biochemical techniques. The results of this study demonstrate that all criteria considered definitive for estrogen receptors can be met by autoradiographic analysis, which makes feasible the assessment of estrogen receptor activity in the individual cell types that comprise fetal and neonatal estrogen target organs.




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Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1983 by The Endocrine Society