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Studies on Cyclic Nucleotides in the Adrenal Gland. XI. Adrenergic Regulation of Adenylate Cyclase Activity in the Adrenal Cortex^*

SUMIO SHIMA, KENJI KOMORIYAMA, MASANAO HIRAI and HIROSHI KOUYAMA

Department of Pharmacology and Radioisotope Institute for Basic Medicine, St. Marianna University School of Medicine Kawasaki City, Kanagawa 213, Japan

Address all correspondence and reprint requests to: Dr. Sumio Shima, Department of Pharmacology, St. Marianna University School of Medicine, Kawasaki City, Kanagawa 213, Japan.

Abstract

Adrenergic regulation of adenylate cyclase activities in the zona glomerulsa (the capsular fraction) and the zona fasciculata-reticularis (the decapsulated fraction) from rat adrenocortical glands has been investigated. Specific binding of [³H]dihydroalprenolol to the membrane from the capsular and the decapsulated fractions was saturable with dissociation constant (K_d) of 4.67 and 5.1 µM, respectively. The receptor density in the capsular and the decapsulated fractions was 230 and 235 fmol/mg protein, respectively. The potencies which isoproterenol, epinephrine, salbutamol, and norepinephrine competed with [³H]dihydroalprenolol binding sites indicted that adrenergic receptors of the capsular and the decapsulated membranes were of the β₂-type. β-Adrenergic stimulation of the adenylate cyclase system was observed only in the capsular fraction. This suggests that β-adrenergic receptors of the capsular membrane are associated with their adenylate cyclase system, but those of the decapsulated membrane are not. Maximum stimulatory concentrations of ACTH and isoproterenol had no additive effect on the capsular adenylate cyclase, indicating that receptors for ACTH and β-adrenergic agonists are coupled to a common pool of the cyclase. (Endocrinology 114: 325, 1984)

Footnotes

^* Presented in part at the 85th International Congress of Pharmacology, Tokyo, Japan 1981.

Postgraduate fellow of the Department of Obstetrics and Gynecology.

Received August 18, 1982.

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