help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leonard, J. L.
Right arrow Articles by Larsen, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leonard, J. L.
Right arrow Articles by Larsen, P. R.

Endocrinology, Vol 114, 998-1004, Copyright © 1984 by Endocrine Society

ARTICLES

Acute posttranscriptional regulation of cerebrocortical and pituitary iodothyronine 5'-deiodinases by thyroid hormone

JL Leonard, JE Silva, MM Kaplan, SA Mellen, TJ Visser and PR Larsen

Cerebrocortical and pituitary iodothyronine 5'-deiodinases produce 50% or more of the T3 found in these tissues and show rapid 3- to 5-fold changes in response to changes in the thyroid status. Since many T3 responses are initiated in the cell nucleus and require protein synthesis, we studied the influence of cycloheximide and actinomycin D on the acute T3 suppression of cerebrocortical and pituitary 5'- deiodinase activities in hypothyroid rats. Cycloheximide inhibited protein synthesis by more than 95%, but did not prevent the rapid (4-h) T3-mediated decreases in 5'-deiodinase activity. In the presence of cycloheximide, T3 decreased enzyme activity by more than 85%, with a t1/2 of approximately 70 min in the cerebral cortex and about 110 min in the pituitary. In vitro, neither T3 nor cycloheximide in large excess had any effect on enzyme activity. In cycloheximide-treated rats, cerebrocortical 5'-deiodinase activity decreased with a fractional turnover rate (k) of 1.2 h-1 in euthyroid and 0.07 h-1 in hypothyroid rats, respectively, with corresponding steady state enzyme levels of 18 +/- 3 and 145 +/- 9 U/mg protein. The resulting production rates of cerebrocortical 5'-deiodinase were 22 and 10 U/mg protein X h. Actinomycin D failed to alter either the cerebrocortical 5'-deiodinase in euthyroid rats in 4 h or the inhibitory effect of T3, but inhibited RNA synthesis by more than 70%. These data indicate that the T3- dependent fall in cerebrocortical 5'-deiodinase is mediated by a posttranscriptional mechanism that ultimately increases the rate of degradation/inactivation of the enzyme.


This article has been cited by other articles:


Home page
 J EndocrinolHome page
M. S Wagner, S. M Wajner, J. M Dora, and A. L. Maia
Regulation of Dio2 gene expression by thyroid hormones in normal and type 1 deiodinase-deficient C3H mice
J. Endocrinol., June 1, 2007; 193(3): 435 - 444.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. P. Farwell, S. A. Dubord-Tomasetti, A. Z. Pietrzykowski, and J. L. Leonard
Dynamic Nongenomic Actions of Thyroid Hormone in the Developing Rat Brain
Endocrinology, May 1, 2006; 147(5): 2567 - 2574.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Morimura, K. Tsunekawa, T. Kasahara, K. Seki, T. Ogiwara, M. Mori, and M. Murakami
Expression of Type 2 Iodothyronine Deiodinase in Human Osteoblast Is Stimulated by Thyrotropin
Endocrinology, April 1, 2005; 146(4): 2077 - 2084.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Yasuzawa-Amano, N. Toyoda, A. Maeda, A. Kosaki, Y. Mori, T. Iwasaka, and M. Nishikawa
Expression and Regulation of Type 2 Iodothyronine Deiodinase in Rat Aorta Media
Endocrinology, December 1, 2004; 145(12): 5638 - 5645.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
F. W. J. S. Wassen, W. Klootwijk, E. Kaptein, D. J. Duncker, T. J. Visser, and G. G. J. M. Kuiper
Characteristics and Thyroid State-Dependent Regulation of Iodothyronine Deiodinases in Pigs
Endocrinology, September 1, 2004; 145(9): 4251 - 4263.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Friedrichsen, S. Christ, H. Heuer, M. K. H. Schafer, A. Mansouri, K. Bauer, and T. J. Visser
Regulation of Iodothyronine Deiodinases in the Pax8-/- Mouse Model of Congenital Hypothyroidism
Endocrinology, March 1, 2003; 144(3): 777 - 784.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. G. J. M. Kuiper, W. Klootwijk, and T. J. Visser
Substitution of Cysteine for a Conserved Alanine Residue in the Catalytic Center of Type II Iodothyronine Deiodinase Alters Interaction with Reducing Cofactor
Endocrinology, April 1, 2002; 143(4): 1190 - 1198.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
A. C. Bianco, D. Salvatore, B. Gereben, M. J. Berry, and P. R. Larsen
Biochemistry, Cellular and Molecular Biology, and Physiological Roles of the Iodothyronine Selenodeiodinases
Endocr. Rev., February 1, 2002; 23(1): 38 - 89.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
H. Mizuma, M. Murakami, and M. Mori
Thyroid Hormone Activation in Human Vascular Smooth Muscle Cells : Expression of Type II Iodothyronine Deiodinase
Circ. Res., February 16, 2001; 88(3): 313 - 318.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. M. Bates, Vickie. L. Spate, J. S. Morris, D. L. St. Germain, and V. A. Galton
Effects of Selenium Deficiency on Tissue Selenium Content, Deiodinase Activity, and Thyroid Hormone Economy in the Rat during Development
Endocrinology, July 1, 2000; 141(7): 2490 - 2500.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Canettieri, F. S. Celi, G. Baccheschi, L. Salvatori, M. Andreoli, and M. Centanni
Isolation of Human Type 2 Deiodinase Gene Promoter and Characterization of a Functional Cyclic Adenosine Monophosphate Response Element
Endocrinology, May 1, 2000; 141(5): 1804 - 1813.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Steinsapir, A. C. Bianco, C. Buettner, J. Harney, and P. R. Larsen
Substrate-Induced Down-Regulation of Human Type 2 Deiodinase (hD2) Is Mediated through Proteasomal Degradation and Requires Interaction with the Enzyme's Active Center
Endocrinology, March 1, 2000; 141(3): 1127 - 1135.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
Y. Hosoi, M. Murakami, H. Mizuma, T. Ogiwara, M. Imamura, and M. Mori
Expression and Regulation of Type II Iodothyronine Deiodinase in Cultured Human Skeletal Muscle Cells
J. Clin. Endocrinol. Metab., September 1, 1999; 84(9): 3293 - 3300.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
J. L. Leonard, D. M. Leonard, M. Safran, R. Wu, M. L. Zapp, and A. P. Farwell
The Mammalian Homolog of the Frog Type II Selenodeiodinase Does Not Encode a Functional Enzyme in the Rat
Endocrinology, May 1, 1999; 140(5): 2206 - 2215.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
S.-W. Kim, J. W. Harney, and P. R. Larsen
Studies of the Hormonal Regulation of Type 2 5'-Iodothyronine Deiodinase Messenger Ribonucleic Acid in Pituitary Tumor Cells Using Semiquantitative Reverse Transcription-Polymerase Chain Reaction
Endocrinology, December 1, 1998; 139(12): 4895 - 4905.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Diano, F. Naftolin, F. Goglia, and T. L. Horvath
Fasting-Induced Increase in Type II Iodothyronine Deiodinase Activity and Messenger Ribonucleic Acid Levels Is Not Reversed by Thyroxine in the Rat Hypothalamus
Endocrinology, June 1, 1998; 139(6): 2879 - 2884.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. A. Burmeister, J. Pachucki, and D. L. St. Germain
Thyroid Hormones Inhibit Type 2 Iodothyronine Deiodinase in the Rat Cerebral Cortex by Both Pre- and Posttranslational Mechanisms
Endocrinology, December 1, 1997; 138(12): 5231 - 5237.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. G. Schuur, F. M. Boekhorst, A. Brouwer, and T. J. Visser
Extrathyroidal Effects of 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on Thyroid Hormone Turnover in Male Sprague-Dawley Rats
Endocrinology, September 1, 1997; 138(9): 3727 - 3734.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
J. H. Oppenheimer and H. L. Schwartz
Molecular Basis of Thyroid Hormone-Dependent Brain Development
Endocr. Rev., August 1, 1997; 18(4): 462 - 475.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. M. Tu, S.-W. Kim, D. Salvatore, T. Bartha, G. Legradi, P. R. Larsen, and R. M. Lechan
Regional Distribution of Type 2 Thyroxine Deiodinase Messenger Ribonucleic Acid in Rat Hypothalamus and Pituitary and Its Regulation by Thyroid Hormone
Endocrinology, August 1, 1997; 138(8): 3359 - 3368.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. B. Becker, K. C. Stephens, J. C. Davey, M. J. Schneider, and V. A. Galton
The Type 2 and Type 3 Iodothyronine Deiodinases Play Important Roles in Coordinating Development in Rana catesbeiana Tadpoles
Endocrinology, July 1, 1997; 138(7): 2989 - 2997.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Orozco, J. E. Silva, and C. Valverde-R
Rainbow Trout Liver Expresses Two Iodothyronine Phenolic Ring Deiodinase Pathways with the Characteristics of Mammalian Types I and II 5'-Deiodinases
Endocrinology, January 1, 1997; 138(1): 254 - 258.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. P. Farwell, M. Safran, S. Dubord, and J. L. Leonard
Degradation and Recycling of the Substrate-binding Subunit of Type II Iodothyronine 5'-Deiodinase in Astrocytes
J. Biol. Chem., July 5, 1996; 271(27): 16369 - 16374.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. M. Leonard, S. J. Stachelek, M. Safran, A. P. Farwell, T. F. Kowalik, and J. L. Leonard
Cloning, Expression, and Functional Characterization of the Substrate Binding Subunit of Rat Type II Iodothyronine 5'-Deiodinase
J. Biol. Chem., August 11, 2000; 275(33): 25194 - 25201.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. Stachelek, T. F. Kowalik, A. P. Farwell, and J. L. Leonard
Myosin V Plays an Essential Role in the Thyroid Hormone-dependent Endocytosis of Type II Iodothyronine 5'-Deiodinase
J. Biol. Chem., October 6, 2000; 275(41): 31701 - 31707.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1984 by The Endocrine Society