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Endocrinology, Vol 115, 317-323, Copyright © 1984 by Endocrine Society

ARTICLES

Enrichment of rat anterior pituitary cell types by metrizamide density gradient centrifugation

RJ Koenig and AY Watson

Anterior pituitary cells derived from hypothyroid rats were enzymatically dispersed and then fractionated by equilibrium sedimentation through a metrizamide density gradient. Populations relatively enriched in thyrotrophs, somatotrophs, mammotrophs, or gonadotrophs were obtained. The thyrotroph-enriched fraction was put into short term culture and tested for hormone responsiveness. TRH stimulated TSH release with an ED50 of 8 X 10(-10) M, and this TRH effect was inhibited by T3 with an ED50 of 1.6 X 10(-11) M free T3. Metrizamide density gradient centrifugation is a useful technique for obtaining relatively enriched populations of viable anterior pituitary cell types.


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A. Alkemade, E. C Friesema, G. G Kuiper, W. M Wiersinga, D. F Swaab, T. J Visser, and E. Fliers
Novel neuroanatomical pathways for thyroid hormone action in the human anterior pituitary.
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