Endocrinology, Vol 115, 330-336, Copyright © 1984 by Endocrine Society

ARTICLES

A monoclonal antibody against a synthetic peptide is specific for the free native human chorionic gonadotropin beta-subunit

D Bellet, JM Bidart, M Jolivet, A Tartar, JM Caillaud, M Ozturk, MC Strugo, F Audibert, H Gras-Masse and M Assicot

A totally synthetic molecule (109-145 peptide) analogous to the beta- subunit carboxyl terminus was used as an antigen in the development of antibodies by the hybridoma technique. A monoclonal antibody (702 D7) specifically recognized the free native beta-human CG (beta hCG). 702 D7 was of the immunoglobulin G1 subclass and was directed against an antigenic site localized in a 10-amino acid sequence (109-118) or less. The recognition of an epitope located in the 109-118 region could explain the specific recognition of beta hCG observed with 702 D7, in contrast to monoclonal antibodies directed against a 118-145 region with a recognition of both beta hCG and whole hCG, as observed with a second monoclonal antibody (1032) to synthetic peptide. Immunohistochemical results and preliminary data obtained from the immunoradiometric assay show that 702 D7 provides a clinical tool for the detection of free beta-subunit secretion even at low concentrations, and could allow the study of this subunit or its metabolites produced by normal and tumoral cells.

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