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Evidence for Only One β-Luteinizing Hormone and No β-Chorionic Gonadotropin Gene in the Rat^*

Department of Biochemistry and Center for Reproductive Sciences, Columbia University College of Physicians and Surgeons New York, New York 10032

Address correspondence and request for reprints to: James L. Roberts, Ph.D., Department of Biochemistry, Columbia University, Center for Reproductive Sciences, 630 West 160th Street, New York, New York, 10032.

Abstract

We have examined the rat genome and placenta for the presence of a mRNA or gene which codes for a protein identical or similar to the β-subunit of LH. A cDNA clone was isolated that encodes for amino acids 44 through 121 of the mature β-subunit of rat LH (rLH). This rβLH cDNA was used as a hybridization probe to study the structure of the gene(s) which encode for the β-subunit of either LH or a LH-like protein in the rat genome. Restriction enzyme digestion analysis of rat genomic DNA using Southern blots revealed only one fragment that hybridized to ³²P-labeled rβLH cDNA. In contrast, restriction enzyme analysis of human and rhesus monkey genomic DNA (known to have both LH and CG) gave multiple fragments which hybridized to a ³²P-labeled human βLH DNA. We have also examined the possibility of a mRNA which can encode the β-subunit of either LH or CG in the rat placenta. Northern blot analysis of total RNA isolated from rat placenta and rat anterior pituitary revealed that only the anterior pituitary contains a mRNA which is complementary to rLH cDNA. These results suggest that: 1) there is only one gene which encodes the β-subunit of LH in the rat haploid genome; 2) there is no gene which encodes for a β-subunit of a CG molecule in the rat; 3) there is no mRNA in the rat placenta which encodes for the β-subunit of LH or an LH-like molecule. (Endocrinology 115: 385–391, 1984)

Footnotes

^* This work was supported by a grant (to J. L. R.) from the Ford, Mellon, and Rockefeller Foundations.

Received November 29, 1983.

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