help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tseng, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tseng, L.

Endocrinology, Vol 115, 833-835, Copyright © 1984 by Endocrine Society

ARTICLES

Effect of estradiol and progesterone on human endometrial aromatase activity in primary cell culture

L Tseng

Isolated human endometrial stromal cells were cultured in RPMI 1640 medium containing insulin, antibiotics and 10% fetal calf serum. After the stromal cells developed a confluent monolayer, estradiol (E2), progesterone (P), or both were separately added to the culture medium and cells were continuously cultured for various periods of time. Aromatase activity was measured in control and hormone-treated cells by incubating the intact cells with [3H]testosterone and isolating the estrogens at the end of incubation. E2 alone did not change the aromatase activity. P caused an 8- to greater than 40-fold increase over the control values (1 to 7 fmol/mg protein X h), and the activity was further increased in the presence of E2 (20- to 100-fold). The stimulation of aromatase activity by P was found to be both time- and dose-dependent and blocked by actinomycin D. Maximal stimulation was reached after the stromal cells were treated with 300 nM P for 3 days. At 30 nM P, a concentration similar to the plasma level during the luteal phase of the menstrual cycle, 80% of maximal stimulation was noted. These results indicate that P stimulates aromatase in endometrial stromal cells and E2 potentiates this stimulation. Much smaller effects of E2 and P on the aromatase activity were noted in endometrial epithelial glands.


This article has been cited by other articles:


Home page
 Hum ReprodHome page
L. Pal, A. L. Niklaus, M. Kim, S. Pollack, and N. Santoro
Heterogeneity in endometrial expression of aromatase in polyp-bearing uteri
Hum. Reprod., January 1, 2008; 23(1): 80 - 84.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
H. Dassen, C. Punyadeera, R. Kamps, B. Delvoux, A. Van Langendonckt, J. Donnez, B. Husen, H. Thole, G. Dunselman, and P. Groothuis
Estrogen metabolizing enzymes in endometrium and endometriosis
Hum. Reprod., December 1, 2007; 22(12): 3148 - 3158.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1984 by The Endocrine Society