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Endocrinology, Vol 115, 836-838, Copyright © 1984 by Endocrine Society

ARTICLES

Effects of in vivo estradiol administration on availability of rat uterine nuclear acceptor in measured in vitro

RS Chuknyiska, M Haji, RH Foote and GS Roth

Estradiol injected in vivo successfully completes for binding to uterine nuclear acceptor sites measured by the assay of Kon and Spelsberg (1). Such competition is time-and dose-dependent, with maximal inhibition (75%) 1 h after injection and at a dose of 10 micrograms of 17 beta-estradiol per 300 g of BW. Thus, this assay appears to accurately measure those uterine nuclear acceptor sites mediating estrogen action in vivo.


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A. H. Lauber, T. J. Barrett, M. Subramaniam, M. Schuchard, and T. C. Spelsberg
A DNA-binding Element for a Steroid Receptor-binding Factor Is Flanked by Dual Nuclear Matrix DNA Attachment Sites in the c-myc Gene Promoter
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[Abstract] [Full Text] [PDF]


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