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Hormonal Regulation of Deoxyribonucleic Acid Polymerase β Activity in Rat Testis^*

Department of Internal Medicine, Fujita-Gakuen University School of Medicine Toyoake, Aichi 470-11 Japan
Department of Biochemistry Aichi Prefecture Colony, Kasugai, Aichi 480-03, Japan
Institute for Developmental Research Aichi Prefecture Colony, Kasugai, Aichi 480-03, Japan

Address requests for reprints to: Akio Nagasaka, M.D., Department of Internal Medicine, Fujita-Gakuen University School of Medicine, Toyoake, Aichi 470-11 Japan.

Abstract

The content of DNA polymerase β in whole rat testes drastically decreased upon hypophysectomy: at 1 and 2 weeks, to 12.5% and 5% of the control testes of sham-operated rats, respectively. DNA polymerase β activity per cell (per milligram DNA) also decreased to 14% of the control within 2 weeks after hypophysectomy. The DNA polymerase in the testis also decreased, but the DNA polymerase was relatively resistant to hypophysectomy. The testicular DNA polymerase β from hypophysectomized rats sedimented at 4.5 S, whereas that from sham-operated controls sedimented at 3.3 S. The reduced level of testicular DNA polymerase β and the change in molecular size induced by hypophysectomy were largely reversed by daily injections of LH and FSH. The reduced activity was also fully reversed by the injection of testosterone. These results suggest that the level of DNA polymerase β in the rat testis depends largely on the level of testosterone produced by Leydig cells in the testis itself, but is also regulated by pituitary gonadotropins. (Endocrinology 115: 1110–1115, 1984)

Footnotes

^* This work was supported by the Fujita-Gakuen Science Foundation and a Grant-in-Aid for Cancer Research from the Ministry of Education, Science, and Culture of Japan.

Received July 21, 1983.

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