help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Danielpour, D.
Right arrow Articles by Sirbasku, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Danielpour, D.
Right arrow Articles by Sirbasku, D. A.

Endocrinology, Vol 115, 1221-1223, Copyright © 1984 by Endocrine Society

ARTICLES

Identification of an autostimulatory growth factor in the extracts and conditioned medium of GH3/C14 rat pituitary cells in culture

D Danielpour, T Ikeda, MW Kunkel and DA Sirbasku

An acid- and heat-stable low molecular weight (Mr 2,500-6,200) autostimulatory growth factor has been identified in extracts of GH3/C14 rat pituitary tumors, in extracts of these cells in culture and in their serum-free conditioned medium. The concentrations of autostimulatory activity required to achieve ED50 in serum-free culture were 4.3, 4.0 and 85 micrograms/ml for extracts of tumors, the cultured cells, and from the conditioned medium of the cells in culture, respectively. To characterize this activity, we have used an isolation method that included 0.1 M acetic acid extraction, heating at 95 C, SP- Sephadex chromatography, and finally, Sephadex G-50 chromatography. The identification of this activity if rapidly growing, relatively autonomous GH3/C14 tumors suggests an autostimulatory role in the conversion from estrogen-responsive growth to autonomy.


This article has been cited by other articles:


Home page
 ScienceHome page
P. Hinkle and P. Kinsella
Thyroid hormone induction of an autocrine growth factor secreted by pituitary tumor cells
Science, December 19, 1986; 234(4783): 1549 - 1552.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1984 by The Endocrine Society