This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by KUDLOW, J. E. Articles by KOBRIN, M. S. Search for Related Content PubMed PubMed Citation Articles by KUDLOW, J. E. Articles by KOBRIN, M. S.

Secretion of Epidermal Growth Factor-Like Mitogens by Cultured Cells from Bovine Anterior Pituitary Glands^*

JEFFREY E. KUDLOW and MICHAEL S. KOBRIN

Departments of Clinical Biochemistry and Medicine, University of Toronto, Banting Institute Toronto, Ontario, Canada M5G1L5

Address request for reprints to: Dr. J. E. Kudlow, Department of Clinical Biochemistry, Banting Institute, Room 414,100 College Street, University of Toronto, Toronto, Ontario, Canada M5G 1L5.

Abstract

We have partially characterized growth factor activity secreted by early cultures of cells derived from bovine calf pituitary glands. Serum-free, chemically defined culture medium conditioned by pituitary cells stimulated DNA synthesis in rat kidney fibroblasts (NRK cells) equivalent to unconditioned medium containing 3 x 10^–10 M epidermal growth factor (EGF). The conditioned medium contained activity which competed with ¹²⁵I-EGF for binding to EGF-receptors which.was also equivalent to competition by 3 x 10^–10 M EGF in a radiore-ceptor assay. Secondary pituitary cultures produced about 10-fold more competing activity than did primary cultures and the production rate of the EGF-competing activity by the secondary cultures remained stable for over 4 weeks. Bio-Gel P-60 chromatography in 1 M acetic acid of an extract of conditioned medium resolved three peaks of EGF-competing activity. These peaks chromatographed with apparent mol wts of 17,000, 9,000, and 6,000. These peaks of activity were not as a result of EGF-binding proteins in the culture medium. The 6,000 mol wt peak contained activity which competed equally well with EGF for EGF receptor and antimouse EGF antibody binding. The 17,000 and 9,000 mol wt activities competed more effectively for binding to receptors than antibodies. The mitogenic activity in the three peaks correlated with the receptor-competing activity. However, the 17,000 and 9,000 mol wt peaks but not the 6,000 mol wt peak contained transforming growth factor activity capable of stimulating NRK cells to assume anchorage independent growth. These results suggest that a subpopulation of cells in pituitary glands secrete EGF and EGF-like transforming growth factors. (Endocrinology 115: 911–917, 1984)

Footnotes

^* This work was supported by grants from the Medical Research Council of Canada and the National Cancer Institute (Canada).

Medical Scholar of the Canadian Life and Health Insurance Association.

Received November 17, 1983.

This article has been cited by other articles:

				M. E. Freeman, B. Kanyicska, A. Lerant, and G. Nagy Prolactin: Structure, Function, and Regulation of Secretion Physiol Rev, October 1, 2000; 80(4): 1523 - 1631. [Abstract] [Full Text] [PDF]

				D. Ray and S. Melmed Pituitary Cytokine and Growth Factor Expression and Action Endocr. Rev., April 1, 1997; 18(2): 206 - 228. [Abstract] [Full Text]