This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by NEMERE, I. Articles by NORMAN, A. W. Search for Related Content PubMed PubMed Citation Articles by NEMERE, I. Articles by NORMAN, A. W.

Calcium Transport in Perfused Duodena from Normal Chicks: Enhancement within Fourteen Minutes of Exposure to 1,25-Dihydroxyvitamin D₃^*

ILKA NEMERE, YOSHIO YOSHIMOTO and ANTHONY W. NORMAN

Department of Biochemistry, University of California Riverside, California 92521

Abstract

The effect of 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃) on calcium transport was studied in vascularly perfused duodena of normal, vitamin D-replete chicks. Addition of 130 pM 1,25(OH)₂D₃ to the perfusate resulted in a significant increase in ⁴⁵Ca transport from the lumen to the vascular effluent within 14 µn; the transport rate rose to 140% of levels in comparable preparations exposed for 40 min to vehicle. No effects of 1,25(OH)₂D₃ were noted on the back flux or transfer of ⁴⁵Ca from the vascular effluent to the lumen. Vascular perfusion with 100 µM colchicine, an antimicrotubular agent, abolished the rapid lumen-to-vascular effluent effect of 1,25(OH)₂D₃ on ⁴⁵Ca transport, relative to preparations exposed to the secosteroid and 100 µM lumicolchicine, (a light inactivated analog of colchicine). Colchicine did not, however, alter basal ⁴⁵Ca transport rates. Addition of 130 pM 1,25(OH)₂D₃ to the lumenal compartment of normal chicks or vascular perfusion of duodena from vitamin D-deficient birds failed to increase ⁴⁵Ca transport above control levels. Perfusion of duodena from normal chicks with 650 pM 1,25(OH)₂D₃ further increased calcium transport to 170% of levels observed in preparations treated with 130 pM steroid, and 210% of levels in controls. Although 15 nM vitamin D₃ had no effect, in one series of experiments 125 nM 25-hydroxyvitamin D₃ elicited vascular calcium levels that were 185% of controls at 40 min. These results suggest that 1,25(OH)₂D₃ can act in vitamin D-replete animals to produce rapid unidirectional calcium transport responses (through unknown mechanisms), as well as by interaction with intestinal nuclear receptors in D-deficient animals to promote induction of protein(s) that support long acting calcium transport responses. (Endocrinology 115: 1476–1483, 1981)

Footnotes

^* This work was supported by USPHS Grants T32-AM-07310 and AM-09012-018. This is paper XLV in a series entitled "Studies on the Mode of Action of Calciferol;" the previous paper in this series is "Intestinal Brush Border Hydrolase Topography: Effects of Vitamin D₃ and Filipin by Nemere, I., C. S. Dunlap, and A. W. Norman, 1983, Biochim. Biophys. Acta 729:35.

Present address: Department of Biology, University of California, San Diego, La Jolla, CA 92093.

Visiting Professor from the 3rd Division of Internal Medicine, Kobe University School of Medicine, Kobe, Japan.

To whom correspondence and requests for reprints should be addressed.

Received November 15, 1983.

This article has been cited by other articles:

				M. T. Mizwicki and A. W. Norman The Vitamin D Sterol-Vitamin D Receptor Ensemble Model Offers Unique Insights into Both Genomic and Rapid-Response Signaling Sci. Signal., June 16, 2009; 2(75): re4 - re4. [Abstract] [Full Text] [PDF]

				A. W. Norman Vitamin D Receptor: New Assignments for an Already Busy Receptor Endocrinology, December 1, 2006; 147(12): 5542 - 5548. [Abstract] [Full Text] [PDF]

				T. I. Ellison, D. R. Dowd, and P. N. MacDonald Calmodulin-Dependent Kinase IV Stimulates Vitamin D Receptor-Mediated Transcription Mol. Endocrinol., September 1, 2005; 19(9): 2309 - 2319. [Abstract] [Full Text] [PDF]

				A. S. Dusso, A. J. Brown, and E. Slatopolsky Vitamin D Am J Physiol Renal Physiol, July 1, 2005; 289(1): F8 - F28. [Abstract] [Full Text] [PDF]

				A. M. Vertino, C. M. Bula, J.-R. Chen, M. Almeida, L. Han, T. Bellido, S. Kousteni, A. W. Norman, and S. C. Manolagas Nongenotropic, Anti-Apoptotic Signaling of 1{alpha},25(OH)2-Vitamin D3 and Analogs through the Ligand Binding Domain of the Vitamin D Receptor in Osteoblasts and Osteocytes: MEDIATION BY Src, PHOSPHATIDYLINOSITOL 3-, AND JNK KINASES J. Biol. Chem., April 8, 2005; 280(14): 14130 - 14137. [Abstract] [Full Text] [PDF]

				J. A. Huhtakangas, C. J. Olivera, J. E. Bishop, L. P. Zanello, and A. W. Norman The Vitamin D Receptor Is Present in Caveolae-Enriched Plasma Membranes and Binds 1{alpha},25(OH)2-Vitamin D3 in Vivo and in Vitro Mol. Endocrinol., November 1, 2004; 18(11): 2660 - 2671. [Abstract] [Full Text] [PDF]

				T.-M. Nguyen, M. Lieberherr, J. Fritsch, H. Guillozo, M. L. Alvarez, Z. Fitouri, F. Jehan, and M. Garabedian The Rapid Effects of 1,25-Dihydroxyvitamin D3 Require the Vitamin D Receptor and Influence 24-Hydroxylase Activity: STUDIES IN HUMAN SKIN FIBROBLASTS BEARING VITAMIN D RECEPTOR MUTATIONS J. Biol. Chem., February 27, 2004; 279(9): 7591 - 7597. [Abstract] [Full Text] [PDF]

				M. van Abel, J. G. J. Hoenderop, A. W. C. M. van der Kemp, J. P. T. M. van Leeuwen, and R. J. M. Bindels Regulation of the epithelial Ca2+ channels in small intestine as studied by quantitative mRNA detection Am J Physiol Gastrointest Liver Physiol, June 9, 2003; 285(1): G78 - G85. [Abstract] [Full Text] [PDF]

				A. L. M. Sutton and P. N. MacDonald Vitamin D: More Than a "Bone-a-Fide" Hormone Mol. Endocrinol., May 1, 2003; 17(5): 777 - 791. [Abstract] [Full Text] [PDF]

				R. G. Erben, D. W. Soegiarto, K. Weber, U. Zeitz, M. Lieberherr, R. Gniadecki, G. Moller, J. Adamski, and R. Balling Deletion of Deoxyribonucleic Acid Binding Domain of the Vitamin D Receptor Abrogates Genomic and Nongenomic Functions of Vitamin D Mol. Endocrinol., July 1, 2002; 16(7): 1524 - 1537. [Abstract] [Full Text] [PDF]

				I. Nemere, R. Ray, and W. McManus Immunochemical studies on the putative plasmalemmal receptor for 1,25(OH)2D3. I. Chick intestine Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E1104 - E1114. [Abstract] [Full Text] [PDF]

				S. Puri, D. D. Bansal, M. R. Uskokovic, and R. R. MacGregor Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)2D3 Am J Physiol Endocrinol Metab, February 1, 2000; 278(2): E293 - E301. [Abstract] [Full Text] [PDF]

				A. J. Brown, A. Dusso, and E. Slatopolsky Vitamin D Am J Physiol Renal Physiol, August 1, 1999; 277(2): F157 - F175. [Abstract] [Full Text] [PDF]

				L. P. Zanello and A. W. Norman Stimulation by 1alpha ,25(OH)2-Vitamin D3 of Whole Cell Chloride Currents in Osteoblastic ROS 17/2.8 Cells. A STRUCTURE-FUNCTION STUDY J. Biol. Chem., September 5, 1997; 272(36): 22617 - 22622. [Abstract] [Full Text] [PDF]

				A. W. Norman, W. H. Okamura, M. W. Hammond, J. E. Bishop, M. C. Dormanen, R. Bouillon, H. van Baelen, A. L. Ridall, E. Daane, R. Khoury, et al. Comparison of 6-s-cis- and 6-s-trans-Locked Analogs of 1{alpha},25-Dihydroxyvitamin D3 Indicates That the 6-s-cis Conformation Is Preferred for Rapid Nongenomic Biological Responses and That Neither 6-s-cis- nor 6-s-trans-Locked Analogs Are Preferred for Genomic Biological Responses Mol. Endocrinol., September 1, 1997; 11(10): 1518 - 1531. [Abstract] [Full Text]

				K. Takeuchi and S. E. Guggino 24R,25-(OH)2 Vitamin D3 Inhibits 1alpha ,25-(OH)2 Vitamin D3 and Testosterone Potentiation of Calcium Channels in Osteosarcoma Cells J. Biol. Chem., December 27, 1996; 271(52): 33335 - 33343. [Abstract] [Full Text] [PDF]