help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martin, T. F.
Right arrow Articles by Kowalchyk, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martin, T. F.
Right arrow Articles by Kowalchyk, J. A.

Endocrinology, Vol 115, 1527-1536, Copyright © 1984 by Endocrine Society

ARTICLES

Evidence for the role of calcium and diacylglycerol as dual second messengers in thyrotropin-releasing hormone action: involvement of Ca+2

TF Martin and JA Kowalchyk

The studies reported here were directed toward establishing the mechanism by which TRH acutely stimulates PRL secretion in GH3 pituitary cells. Studies of TRH stimulation of PRL secretion were conducted on a time scale which enables comparison with other reported rapid effects of TRH on GH3 cells. TRH stimulation of secretion was found to be extremely rapid in onset (less than or equal to 10 sec) and biphasic (phase I, 0-2 min; phase II, 5-60 min). The earliest (phase I) secretory response was observed to be independent of medium Ca+2 concentration or Ca+2 influx, but to be dependent on an intracellular Ca+2 pool. The phase II response to TRH was found to depend, in part, on medium Ca+2. The phase I response to TRH could be mimicked only by agents known to influence Ca+2 translocation in GH3 cells (60 mM K+, A23187, ionomycin, carbonyl cyanide p-trifluoromethoxyphenylhydrazone, and carbonyl cyanide m-chlorophenylhydrazone). These agents failed to promote sustained PRL release characteristic of phase II. It is concluded that the ability of TRH to rapidly stimulate PRL secretion (phase I) is correlated with its ability to rapidly promote a transient cytoplasmic Ca+2 concentration rise from an intracellular Ca+2 pool.


This article has been cited by other articles:


Home page
 EndocrinologyHome page
B. Nguyen, M. E. Carbajal, and M. L. Vitale
Intracellular Mechanisms Involved in Dopamine-Induced Actin Cytoskeleton Organization and Maintenance of a Round Phenotype in Cultured Rat Lactotrope Cells
Endocrinology, August 1, 1999; 140(8): 3467 - 3477.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
M. E. Carbajal and M. L. Vitale
The Cortical Actin Cytoskeleton of Lactotropes as an Intracellular Target for the Control of Prolactin Secretion
Endocrinology, December 1, 1997; 138(12): 5374 - 5384.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Yajima, K. Uchino, H. Ito, and S. Kawashima
Mastoparan-Stimulated Prolactin Secretion in Rat Pituitary GH3 Cells Involves Activation of Gq/11 Proteins
Endocrinology, May 1, 1997; 138(5): 1949 - 1958.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1984 by The Endocrine Society