This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by CHAMBERS, T. J. Articles by FULLER, K. Search for Related Content PubMed PubMed Citation Articles by CHAMBERS, T. J. Articles by FULLER, K.

The Effect of Calcium-Regulating Hormones and Prostaglandins on Bone Resorption by Osteoclasts Disaggregated from Neonatal Rabbit Bones^*

T. J. CHAMBERS, P. M. J. McSHEEHY, B. M. THOMSON and K. FULLER

Department of Histopathology, St. Bartholomew’s Hospital West Smithfield, London EC1A7BE, United Kingdom

Abstract

It has previously only been possible to assess osteoclastic bone resorption in intact bone, where other cell types may modify or mediate osteoclastic responses to environmental agents. We have recently developed techniques which enable us to measure bone resorption by osteoclasts extracted from bone and have used these techniques to assess the effects of prostaglandins (PGs) and calcium-regulating hormones on bone resorption by these cells. Osteoclasts were mechanically disaggregated from neonatal rabbit long bones and cultured on slices of devitalized cortical bone for 8 or 24 h. After this time, osteoclasts were associated with the appearance in the scanning electron microscope of characteristic resorption pits, the volume of which was calculated by computer-assisted morphometric and stereophotogrammetric techniques after removal of cells. Salmon calcitonin inhibited osteoclastic bone resorption at concentrations of 1 pg/ml and above, while PTH and 1,25-dihydroxyvitamin D₃ were without significant effect. This suggests that the latter hormones do not increase bone resorption in intact bone through a direct effect on osteoclasts. PGI₂, PGE₁, and PGE₂, all of which are known to stimulate resorption when added to intact bone, paradoxically reduced resorption in our cultures. It appears likely that PGs act as direct inhibitors of osteoclastic bone resorption but have an additional effect on other cells in bone, which are induced by PGs to cause osteoclastic stimulation. (Endocrinology 60: 234–239, 1985)

Footnotes

^* This work was supported by the Medical Research Council, the Wellcome Trust, and the North East Thames Regional Health Authority.

To whom requests for reprints should be addressed.

Received April 4, 1984.

This article has been cited by other articles:

				K. Fuller, B. Kirstein, and T. J. Chambers Murine Osteoclast Formation and Function: Differential Regulation by Humoral Agents Endocrinology, April 1, 2006; 147(4): 1979 - 1985. [Abstract] [Full Text] [PDF]

				F. Okamoto, H. Kajiya, H. Fukushima, E. Jimi, and K. Okabe Prostaglandin E2 activates outwardly rectifying Cl- channels via a cAMP-dependent pathway and reduces cell motility in rat osteoclasts Am J Physiol Cell Physiol, July 1, 2004; 287(1): C114 - C124. [Abstract] [Full Text] [PDF]

				T. Hirayama, L. Danks, A. Sabokbar, and N. A. Athanasou Osteoclast formation and activity in the pathogenesis of osteoporosis in rheumatoid arthritis Rheumatology, November 1, 2002; 41(11): 1232 - 1239. [Abstract] [Full Text] [PDF]

				K. Fuller, C. Murphy, B. Kirstein, S. W. Fox, and T. J. Chambers TNF{alpha} Potently Activates Osteoclasts, through a Direct Action Independent of and Strongly Synergistic with RANKL Endocrinology, March 1, 2002; 143(3): 1108 - 1118. [Abstract] [Full Text] [PDF]

				J. M. Lean, K. Fuller, and T. J. Chambers FLT3 ligand can substitute for macrophage colony-stimulating factor in support of osteoclast differentiation and function Blood, November 1, 2001; 98(9): 2707 - 2713. [Abstract] [Full Text] [PDF]

				M. Bhandari, A. Adili, and E. H. Schemitsch The Efficacy of Low-Pressure Lavage with Different Irrigating Solutions to Remove Adherent Bacteria from Bone J. Bone Joint Surg. Am., March 1, 2001; 83(3): 412 - 412. [Abstract] [Full Text]

				H. Mano, C. Kimura, Y. Fujisawa, T. Kameda, M. Watanabe-Mano, H. Kaneko, T. Kaneda, Y. Hakeda, and M. Kumegawa Cloning and Function of Rabbit Peroxisome Proliferator-activated Receptor delta /beta in Mature Osteoclasts J. Biol. Chem., March 10, 2000; 275(11): 8126 - 8132. [Abstract] [Full Text] [PDF]

				N. M. PEET, P. S. GRABOWSKI, I. LAKETIC-LJUBOJEVIC, and T. M. SKERRY The glutamate receptor antagonist MK801 modulates bone resorption in vitro by a mechanism predominantly involving osteoclast differentiation FASEB J, December 1, 1999; 13(15): 2179 - 2185. [Abstract] [Full Text]

				M. R. Wani, K. Fuller, N. S. Kim, Y. Choi, and T. Chambers Prostaglandin E2 Cooperates with TRANCE in Osteoclast Induction from Hemopoietic Precursors: Synergistic Activation of Differentiation, Cell Spreading, and Fusion Endocrinology, April 1, 1999; 140(4): 1927 - 1935. [Abstract] [Full Text]

				A.T. Vernillo and B.R. Rifkin Effects of Tetracyclines on Bone Metabolism Advances in Dental Research, November 1, 1998; 12(1): 56 - 62. [Abstract] [PDF]

				K. Fuller, B. Wong, S. Fox, Y. Choi, and T. J. Chambers TRANCE Is Necessary and Sufficient for Osteoblast-mediated Activation of Bone Resorption in Osteoclasts J. Exp. Med., September 7, 1998; 188(5): 997 - 1001. [Abstract] [Full Text] [PDF]

				F. Naro, M. Perez, S. Migliaccio, D. L. Galson, P. Orcel, A. Teti, and S. R. Goldring Phospholipase D- and Protein Kinase C Isoenzyme-Dependent Signal Transduction Pathways Activated by the Calcitonin Receptor Endocrinology, July 1, 1998; 139(7): 3241 - 3248. [Abstract] [Full Text] [PDF]

				L. J. Suva, M. S. Flannery, M. P. Caulfield, D. M. Findlay, H. Jüppner, S. R. Goldring, M. Rosenblatt, and M. Chorev Design, Synthesis and Utility of Novel Benzophenone-Containing Calcitonin Analogs for Photoaffinity Labeling the Calcitonin Receptor J. Pharmacol. Exp. Ther., November 1, 1997; 283(2): 876 - 884. [Abstract] [Full Text]

				S. Meghji, P. A. White, S. P. Nair, K. Reddi, K. Heron, B. Henderson, A. Zaliani, G. Fossati, P. Mascagni, J. F. Hunt, et al. Mycobacterium tuberculosis Chaperonin 10 Stimulates Bone Resorption: A Potential Contributory Factor in Pott's Disease J. Exp. Med., October 20, 1997; 186(8): 1241 - 1246. [Abstract] [Full Text] [PDF]

				S. Roux, F. Pichaud, J. Quinn, A. Lalande, C. Morieux, A. Jullienne, and M.-C. de Vernejoul Effects of Prostaglandins on Human Hematopoietic Osteoclast Precursors Endocrinology, April 1, 1997; 138(4): 1476 - 1482. [Abstract] [Full Text] [PDF]

				N. A. ATHANASOU Current Concepts Review - Cellular Biology of Bone-Resorbing Cells J. Bone Joint Surg. Am., July 1, 1996; 78(7): 1096 - 1112. [Full Text]

				A. Teti, R. Paniccia, and S. R. Goldring Calcitonin Increases Cytosolic Free Calcium Concentration via Capacitative Calcium Influx J. Biol. Chem., July 14, 1995; 270(28): 16666 - 16670. [Abstract] [Full Text] [PDF]

				K Fuller and T. Chambers Localisation of mRNA for collagenase in osteocytic, bone surface and chondrocytic cells but not osteoclasts J. Cell Sci., January 6, 1995; 108(6): 2221 - 2230. [Abstract] [PDF]

				D. E. Wolfman and S. P. Tinling Systemic Osteoclast Activation by Localized Pressure Arch Otolaryngol Head Neck Surg, February 1, 1993; 119(2): 165 - 168. [Abstract] [PDF]

				K. Adachi, R. A. Chole, and J. Yee Indomethacin Inhibition of Middle Ear Bone Resorption Arch Otolaryngol Head Neck Surg, March 1, 1991; 117(3): 267 - 269. [Abstract] [PDF]

				G. D. Roodman Osteoclast Differentiation Critical Reviews in Oral Biology & Medicine, January 1, 1991; 2(3): 389 - 409. [Abstract] [Full Text] [PDF]