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Fetal Rat Hyperinsulinism and Hyperglucagonism: Effects on Hepatic Ketogenesis, Lipogenesis, and Gluconeogenesis^*

University of Rochester Medical Center Rochester, New York 14642

Address requests for reprints to: Kenneth McCormick, M.D., Pediatric Endocrinology, Box 777, Strong Memorial Hospital, University of Rochester, 601 Elmwood Avenue, Rochester, New York 14642.

Abstract

To study the direct effects of hyperglucagonism and hyperinsulinism (both with glucose excess) on fetal intermediary metabolism, rat liver explants from 19-day-gestated fetuses were maintained in culture for 48 h. The liver cubes were exposed to 0, 250, or 500 mU/ml porcine insulin or 5 Mg/ml glucagon. In addition, lipogenesis from ³H₂O was cumulated throughout the 48 h. Chronic hyperinsulinism in the fetal rat doubled hepatic lipogenesis and curtailed hepatic gluconeogenesis and ketogenesis by 80% and 50%, respectively. Chronic hyperglucagonism was without effect; however, the fetal liver did yet respond to 1 mM (Bu)₂cAMP. (Endocrinology 116: 1281- 1287,1985)

Footnotes

^* This work was supported by NIH Grant AM-01129 and the Kroc Foundation.

Received May 2, 1984.