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Institute for Cancer Research and Department of Pathology, Osaka University Medical School Nakanoshima 4-3-57, Kita-ku, Osaka, 530, Japan
Address requests for reprints to: Yukihiko Kitamura, M.D. Institute for Cancer Research, Osaka University Medical School, Nakanoshima 4-3-57, Kita-ku, Osaka, 530 Japan.
Abstract
When the uptake of 5-[125I]iodo-2'-deoxyuridine ([125I]IdUrd) into the seminal vesicle of castrated mice was measured 3 days after starting injections of various doses of testosterone propionate (TP), logarithmic values of [125I]IdUrd uptake were proportional to the logarithmic doses of TP in the range of 0.04–2 µg/g BW. The [125I]IdUrd uptake values correlated well with the labeling and mitotic indices of epithelial cells. Since daily injections of 0.4 µgTP/g BW did not increase significantly the weight or DNA content or protein content of the seminal vesicle, the [128I]IdUrd uptake is a sensitive index of androgen action. Moreover, this suggests that low doses of androgen induce division of epithelial cells without resulting in the increase in cell number. The [125I]IdUrd radioactivity in the seminal vesicle was measured 2–15 days after the injection of [125I]IdUrd, since the value represented the fraction of surviving cells synthesizing DNA at the time of [125I]IdUrd injection. When injections of 4 µgTP/g BW were continued, the incorporated radioactivity was retained. In contrast, continuous injections of 0.2 µgTP/g BW did not maintain the radioactivity, of which incorporation was induced by the same dose of TP. Thus, the present result suggests the presence of heterogeneity in androgen-responsive epithelial cells of the seminal vesicle. (Endocrinofogy 116: 1466–1472,1985
Footnotes
* This work was supported by grants from the Ministry of Education, Science and Culture (Cancer Research and Special Project Research), the Ministry of Health and Welfare (Research for Intractable Diseases), the Princess Takamatsu Cancer Research Fund, and the Research Foundation for Multi-disciplinary Cancer Treatment.
Received March 27, 1984.
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