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Prenatal Diethylstilbestrol Exposure Alters Murine Uterine Responses to Prepubertal Estrogen Stimulation^*

Department of Obstetrics and Gynecology, Duke University Medical Center Durham, North Carolina 27710;
The Developmental Endocrinology and Pharmacology Section, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health Research Triangle Park, North Carolina 27709

Address all correspondence and requests for reprints to: Dr. John A. McLachlan, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, P.O. Box 12233, Research Triangle Park, North Carolina 27709.

Abstract

vPrepubertal estrogen stimulation was used to investigate the effects of prenatal diethylstilbestrol (DES) exposure on subsequent growth, secretory activity, and cellular differentiation of the mouse uterus in vivo. Secretory activity was examined using sensitive silver staining of two-dimensional gel electrophoresis of uterine luminal fluid (ULF). Decreased uterine growth response, decreases in ULF quantity and protein concentration, alterations in specific ULF proteins, and altered cellular differentiation were found. This system provides a method for evaluation of the effects of prenatal exposure to DES or other compounds on the estrogen-induced secretory activity of the uterus. The alterations found in this study may be partially responsible for the decreased fertility in this mouse model and may have implication for DES-exposed women. (Endocrinology 116: 1878–1886,1985)

Footnotes

^* This work was supported in part by a grant from the Charles Josiah Trent Foundation (to D.B.M.).

Received August 10, 1984.

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